Techniques to Analyze sRNA Protein Cofactor Self-Assembly In Vitro

Valeria Militello, Sylvain Trépout, Thomas Bizien, Antoine Malabirade, David Partouche, Sergio Marco, Frank Wien, Christophe Sandt, Véronique Arluison, Marisela Velez

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)


Post-transcriptional control of gene expression by small regulatory noncoding RNA (sRNA) needs protein accomplices to occur. Past research mainly focused on the RNA chaperone Hfq as cofactor. Nevertheless, recent studies indicated that other proteins might be involved in sRNA-based regulations. As some of these proteins have been shown to self-assemble, we describe in this chapter protocols to analyze the nano-assemblies formed. Precisely, we focus our analysis on Escherichia coli Hfq as a model, but the protocols presented here can be applied to analyze any polymer of proteins. This chapter thus provides a guideline to develop commonly used approaches to detect prokaryotic protein self-assembly, with a special focus on the detection of amyloidogenic polymers.
Original languageEnglish
Title of host publicationMethods in Molecular Biology - Bacterial Regulatory RNA
Number of pages20
Publication statusPublished - 2018

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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