Systemic Immune Responses in Alzheimer’sDisease: In Vitro Mononuclear CellActivation and Cytokine Production

Anna Di Prima, Matteo Bulati, Domenico Lio, Sonya Vasto, Gabriella Misiano, Giuseppina Colonna Romano, Calogero Caruso, Mario Barbagallo, Giuseppina Candore, Domenico Nuzzo, Pasquale Picone, Marta Di Carlo, Silvio Buffa, Mariavaleria Pellicano'

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51 Citations (Scopus)

Abstract

To investigate the systemic signs of immune-inflammatory responses in Alzheimer’s disease (AD), in the present studywe have analyzed blood lymphocyte subsets and the expression of activation markers on peripheral blood mononuclear cells(PBMCs) fromADpatients and age-matched healthy controls (HC) activated in vitro by recombinant amyloid-β peptide (rAβ42).Our study of AD lymphocyte subpopulations confirms the already described decrease of the absolute number and percentage ofB cells when compared to HC lymphocytes, whereas the other subsets are not significantly different in patients and controls.We report the increased expression of the activation marker CD69 and of the chemokine receptors CCR2 and CCR5 on T cellsbut no changes of CD25 after activation. B cells are also activated by rAβ42 as demonstrated by the enhanced expression ofCCR5. Moreover, rAβ42 induces an increased expression of the scavenger receptor CD36 on monocytes. Some activationmarkers and chemokine receptors are overexpressed in unstimulated AD cells when compared to controls. This is evidence ofthe pro-inflammatory status of AD. Stimulation by rAβ42 also induces the production of the pro-inflammatory cytokines IL-1β,IL-6, IFN-γ, and TNF-α, and of the anti-inflammatory cytokines IL-10 and IL-1Ra. The chemokines RANTES, MIP-1β, andeotaxin as well as some growth factors (GM-CSF, G-CSF) are also overproduced by AD-derived PBMC activated by rAβ42.These results support the involvement of systemic immunity in AD patients. However, our study is an observational one so wecannot draw a conclusion about its contribution to the pathophysiology of the disease.
Original languageEnglish
Pages (from-to)181-192
Number of pages12
JournalJournal of Alzheimer's Disease
Volume21
Publication statusPublished - 2010

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Clinical Psychology
  • Geriatrics and Gerontology
  • Psychiatry and Mental health

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