Survival of Bothryosphaeriaceae species after hot water treatment

Research output: Contribution to conferenceOtherpeer-review

Abstract

The use of Hot Water Treatment (HWT) in the grapevine propagation process has been shown to be A potenzially effective tool to control "Petri" and "Black foot" fungal pathogens. However, effects of HWT on Bothryosphaeriaceae after HWT in two different experiments. First, mycelial plugs contained in Eppendorf tubes with sterile distilled water were subjected to different combination of temperature (50-54°C) and exposure time (!5- 30- 45 minutes)in a hot water bath.In a second trial, the fungi were inoculated into 110 Richter rootstock canes, prviously subjected to HWT. Inoculated canes were incubated at 25°C for three weeks to allow for fungal colonization abd then were subjected at HWT in the range 50 - 53°C for 30 minutes. Survival of fungi after HWT was assessed in both trials. In addition growth rates of treated mycelia were compared to untreated controls. Significant differences in survival and growth for all factors (species, temperature an time)and their interactions were observed in the in vitro assay. Diplodia seriata, Dothiorella viticola, Neofusicoccum luteum and N. parvum, were the most susceptible species to temperature, while Lasiodiplodia sp. and N. vitifusiforme were the most tollerant. In planta experiment, all species sharply reduced their survival after 30 minutes at 51°C. At 50°C. Lasiodiplodia sp. was the most tolerant taxon whereas N. luteum was the most susceptible. These results demonstrate the feasibility of controlling this pathogens by HWT
Original languageEnglish
Pages56-56
Number of pages1
Publication statusPublished - 2014

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