The aim of the following study is to identify and quantify microorganismsin the saliva of black stain patients using species-specific probes for Actinomycesisraelii, Actinomyces naeslundii, Streptococcus mutans and Lactobacillusspp, as well as a universal probe for bacterial 16S rDNA, to obtainthe figure of the total bacterial counts (for test and control group). This studyrepresents a first phase of the research line on extrinsic tooth pigmentationon developmental age patients.Materials and methods. The selected sample is composed of 20 subjectsaged between 2 and 15 years and in good general and oral health among thepatients included in a dental examination trial at the Department of PediatricDentistry of the University Hospital of Palermo, after obteining informedconsent from parents. Subjects with a positive anamnesis for systemic diseasesor with enamel defects were excluded from the study. Exclusion criteriawere also a diet including regular use of tea, coffee, iron supplements,or antibiotic therapies in the 3 months prior to enrollment and the use ofmouthwashes/toothpastes containing antiseptics.Parents/tutors were asked to complete a questionnaire on their occupationalstatus and level of education, as well as their eating habits, the frequencyof brushing and the patient's dental records. A sample of 20 patientswithout BS was used as control group. From all subjects participating to thestudy a sample of 5ml of unstimulated saliva was recovered from the rightand left buccal area through a sterile syringe, after 2 minutes waiting for theaccumulation of an adequate amount of saliva.Statistical analysis was carried out using descriptive and inferential statistics(mean and standard deviation; chi-square test, t test for non-paireddata). StatView 5.0.1 statistical software (SAS Institute, Cary, NC, USA) wasused for this purpose, and the tests were considered significant at p valuesless than or equal to 0.05.The study protocol will be presented for approval to the Palermo 1 ethicscommittee.
|Title of host publication||1a Giornata delle Scuole di Specializzazione "Restate al Di.Chir.On.S." - Principali Linee di Ricerca delle Scuole di Specializzazione|
|Number of pages||2|
|Publication status||Published - 2019|
|Name||ATTI E CONVEGNI|
Giuliana, G., Giammanco, G., Giammanco, M., & Pizzo, G. (2019). Study of oral microbiota and diet as casual factors of Black Stains in the pediatric patient. In 1a Giornata delle Scuole di Specializzazione "Restate al Di.Chir.On.S." - Principali Linee di Ricerca delle Scuole di Specializzazione (pp. 51-52). (ATTI E CONVEGNI).