Studio dell'attività biologica di metaboliti di Citrus spp. su Ceratitis capitata

Maurizio Bruno, Virgilio Caleca, Nicoletta Faraone

Research output: Contribution to conferenceOther

Abstract

[automatically translated] The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera Tephritidae) is one of the most harmful insects in the world. In recent years, numerous researches have been conducted on semiochemicals involved in plant-insect host interactions, making use of electrophysiological and behavioral studies, with the aim of using the biologically active substances that may be identified in innovative and sustainable control strategies. It is known that lemons are not attacked by C. capitata, most likely because of the essential epicarp, toxic oils for other fruit flies. In the present paper were performed electrophysiological recordings to assess the sensitivity of the females of C. capitata extracts and oils to the skin of two cultivars (cv.) Sicilian (Interdonato, Meet) Citrus limon Burm. Were conducted, in addition, behavioral bioassays to study the possible biological activity. The perception of the epicarp extracts (in petroleum ether, dichloromethane and methanol) has been studied, in various concentrations, by using a single cell recording technique (SCR), stimulating the tarsal sensilla taste of C. capitata. the responses of different types of taste cells have been recorded. The most intense stimulation was induced by extracts of cv. Interdonato, in particular methanol from the extract, with significant increases in the frequency of action potentials with increasing dose. The perception of the volatile compounds of essential oils extracted from the same cv, in addition to those of the other two CVV. lemon (Monachello, Femminello) It was investigated by elettroantennografica technique (EAG). The EAG Data showed a high sensitivity (about 8.5 mV) of the antennae of C. capitata and a clear dose-response relationship. Behavioral responses of females (virgins and coupled) to epicarp extracts have been studied by means of a double-choice bioassay, using yellow balls (7.0 cm diameter) placed in a special cage. Preliminary tests conducted with three extracts of the CVV. Interdonato and Lunario have provided very promising results. It was recorded, in fact, a general decrease dell'ovideposizione on the balls treated compared to the control Behavioral responses of females (virgins and coupled) to epicarp extracts have been studied by means of a double-choice bioassay, using yellow balls (7.0 cm diameter) placed in a special cage. Preliminary tests conducted with three extracts of the CVV. Interdonato and Lunario have provided very promising results. It was recorded, in fact, a general decrease dell'ovideposizione on the balls treated compared to the control Behavioral responses of females (virgins and coupled) to epicarp extracts have been studied by means of a double-choice bioassay, using yellow balls (7.0 cm diameter) placed in a special cage. Preliminary tests conducted with three extracts of the CVV. Interdonato and Lunario have provided very promising results. It was recorded, in fact, a general decrease dell'ovideposizione on the balls treated compared to the control
Original languageItalian
Pages174-174
Number of pages1
Publication statusPublished - 2011

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