Structural characterization of polysaccharides of a productive strain of the culinary-medicinal king oyster mushroom, pleurotus eryngii (Agaricomycetes), from Italy

Giuseppe Bonaventura, Roberta Calvo, Giuseppe Venturella, Celeste Caruso Bavisotto, Marina Zacchigna, Giuseppe Procida, Francesca Cateni

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6 Citations (Scopus)

Abstract

A preliminary biological investigation of the dry basidiomata of strain C-142-c of Pleurotus eryngii has shown significant antioxidant activity. Two different polysaccharides (PEPS-A1 and PEPS-A2) were isolated from the cultivated edible mushroom, P. eryngii C-142-c strain. Based on acid hydrolysis, methylation analysis, and nuclear magnetic resonance experiments (1H,13C, distortionless enhancement by polarization transfer, double quantum filtered correlation spectroscopy, total correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear single-quantum correlation spectroscopy, and heteronuclear multiple-bond correlation spectroscopy), the structures of the repeating unit of PEPS-A1 and PEPS-A2 were established as follows: (1) PEPS-A1 (α-glucan): [→6)-α-D-Glcp-(1→6)-α-D-Glcp-(1→]n; and (2) PEPS-A2 (β-glucan): [→6)-β-D-Glcp-(1→6)-β-D-Glcp-(1→]n. The antioxidant activity of PEPS-A1 and PEPS-A2 was evaluated as hydroxyl radical scavenging activity. PEPS-A1 and PEPS-A2 showed SC50 values of 400 µg/mL and 122 µg/mL, respectively, suggesting their possible use as a dietary supplement in functional foods. The polysaccharides were tested for their activity on cell viability using a colorectal adenocarcinoma cell line (HT-29). Both polysaccharides affected cell viability after 48 and 72 hours of treatment, inducing the death of 50% of HT-29 cells between 0.25 and 1 µg/mL and between 0.5 and 1 µg/mL, respectively, for PEPS-A1 and PEPS-A2. These results are promising for future applications of these mushroom-derived polysaccharides as antioxidants and antitumor agents.
Original languageEnglish
Pages (from-to)717-726
Number of pages10
JournalInternational Journal of Medicinal Mushrooms
Volume20
Publication statusPublished - 2018

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Pharmacology
  • Drug Discovery

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