Spin trapping as tool for investigating radiation induced free radicals in biologically active molecules

Research output: Contribution to conferenceOther

Abstract

Since from the second half of the last century the spin trapping has found wide applications inchemistry, biology and medicine [1]. Of particular interest are the studied of the free radicals inducedin biologically active molecules, such as DNA and proteins, when they are exposed to ionizingradiation. Their characterization allows to investigate the radiation-induced primary processes insideliving cells. Indeed, ionizing radiations produce many kinds of free radicals through direct action;radicals can be formed also by indirect actions. However, the direct detection of these radicals is hardto obtain as they have a too short lifetime and this makes difficult to record them by conventionalelectron spin resonance (ESR) technique. This limitation can be overcome by means of spin-trappingmethod wherein these short-lived free radicals are converted using a diamagnetic scavenger (thespin trap) to longer-lived radicals (the spin adducts), which can be conveniently investigated by meansof ESR.Here, we review a combined method of ESR and spin trapping to identify free radicals induced bydirect ionization as well as OH-radical reactions in nucleic-acid related and protein-relatedcompounds. ESR combined with spin trapping remains a useful method for clarifying these processesin a living organism.
Original languageEnglish
Number of pages1
Publication statusPublished - 2017

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free radicals
trapping
radiation
molecules
proteins
spin resonance
nucleic acids
biology
medicine
organisms
ionizing radiation
adducts
deoxyribonucleic acid
traps
ionization
life (durability)
cells

Cite this

@conference{a111263a099e492aa0f131e249d7d03a,
title = "Spin trapping as tool for investigating radiation induced free radicals in biologically active molecules",
abstract = "Since from the second half of the last century the spin trapping has found wide applications inchemistry, biology and medicine [1]. Of particular interest are the studied of the free radicals inducedin biologically active molecules, such as DNA and proteins, when they are exposed to ionizingradiation. Their characterization allows to investigate the radiation-induced primary processes insideliving cells. Indeed, ionizing radiations produce many kinds of free radicals through direct action;radicals can be formed also by indirect actions. However, the direct detection of these radicals is hardto obtain as they have a too short lifetime and this makes difficult to record them by conventionalelectron spin resonance (ESR) technique. This limitation can be overcome by means of spin-trappingmethod wherein these short-lived free radicals are converted using a diamagnetic scavenger (thespin trap) to longer-lived radicals (the spin adducts), which can be conveniently investigated by meansof ESR.Here, we review a combined method of ESR and spin trapping to identify free radicals induced bydirect ionization as well as OH-radical reactions in nucleic-acid related and protein-relatedcompounds. ESR combined with spin trapping remains a useful method for clarifying these processesin a living organism.",
author = "Maurizio Marrale and Giorgio Collura and Anna Longo and Salvatore Gallo",
year = "2017",
language = "English",

}

TY - CONF

T1 - Spin trapping as tool for investigating radiation induced free radicals in biologically active molecules

AU - Marrale, Maurizio

AU - Collura, Giorgio

AU - Longo, Anna

AU - Gallo, Salvatore

PY - 2017

Y1 - 2017

N2 - Since from the second half of the last century the spin trapping has found wide applications inchemistry, biology and medicine [1]. Of particular interest are the studied of the free radicals inducedin biologically active molecules, such as DNA and proteins, when they are exposed to ionizingradiation. Their characterization allows to investigate the radiation-induced primary processes insideliving cells. Indeed, ionizing radiations produce many kinds of free radicals through direct action;radicals can be formed also by indirect actions. However, the direct detection of these radicals is hardto obtain as they have a too short lifetime and this makes difficult to record them by conventionalelectron spin resonance (ESR) technique. This limitation can be overcome by means of spin-trappingmethod wherein these short-lived free radicals are converted using a diamagnetic scavenger (thespin trap) to longer-lived radicals (the spin adducts), which can be conveniently investigated by meansof ESR.Here, we review a combined method of ESR and spin trapping to identify free radicals induced bydirect ionization as well as OH-radical reactions in nucleic-acid related and protein-relatedcompounds. ESR combined with spin trapping remains a useful method for clarifying these processesin a living organism.

AB - Since from the second half of the last century the spin trapping has found wide applications inchemistry, biology and medicine [1]. Of particular interest are the studied of the free radicals inducedin biologically active molecules, such as DNA and proteins, when they are exposed to ionizingradiation. Their characterization allows to investigate the radiation-induced primary processes insideliving cells. Indeed, ionizing radiations produce many kinds of free radicals through direct action;radicals can be formed also by indirect actions. However, the direct detection of these radicals is hardto obtain as they have a too short lifetime and this makes difficult to record them by conventionalelectron spin resonance (ESR) technique. This limitation can be overcome by means of spin-trappingmethod wherein these short-lived free radicals are converted using a diamagnetic scavenger (thespin trap) to longer-lived radicals (the spin adducts), which can be conveniently investigated by meansof ESR.Here, we review a combined method of ESR and spin trapping to identify free radicals induced bydirect ionization as well as OH-radical reactions in nucleic-acid related and protein-relatedcompounds. ESR combined with spin trapping remains a useful method for clarifying these processesin a living organism.

UR - http://hdl.handle.net/10447/241113

UR - http://www.chimica.unipd.it/spin-2017/index.php

M3 - Other

ER -