Self-assembled PAA-based nanoparticles as potential gene and protein delivery systems

Nicolò Mauro, Emma Carter, Simon C. W. Richardson, Damien M. Murphy, Paul Dyer, Nicolo Mauro, Paolo Ferruti, Peter C. Griffiths

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

A series of nanoparticles is prepared via layer-by-layer assembly of oppositely charged, synthetic biocompatible polyamidoamine polymers as potential carriers. Particle size, surface charge and internal chain mobility are quantified as a function of the polymer type and number of layers. The effect of addition of surfactant is examined to simulate the effects of nanoparticle dissolution. The cyctotoxicity of these particles (in epithelia and murine cell lines) are orders of magnitude lower than polyethyleneimine controls. Stable nanoparticles may be prepared from mixtures of strongly, oppositely charged polymers, but less successfully from weakly charged polymers, and, given their acceptable toxicity characteristics, such modularly designed constructs show promise for drug and gene delivery. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Original languageEnglish
Pages (from-to)641-649
Number of pages9
JournalMacromolecular Bioscience
Volume13
Publication statusPublished - 2013

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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