Seeding nerve sutures with minced nerve-graft (MINE-G): a simple method to improve nerve regeneration in rats

Francesco Moschella, Anna Martorana, Giovanni Cassata, Carla Cannizzaro, Adriana Cordova, Salvatore D'Arpa, Fulvio Plescia, Giovanni Zabbia, Giovanni Cassata, Roberto Puleio, Luca Cicero, Grazia Salimbeni, Salvatore D’Arpa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: The aim of this study was to assess the effect of seeding the distal nerve suture with nerve fragments in rats. Methods: On 20 rats, a 15 mm sciatic nerve defect was reconstructed with a nerve autograft. In the Study Group (10 rats), a minced 1 mm nerve segment was seeded around the nerve suture. In the Control Group (10 rats), a nerve graft alone was used. At 4 and 12 weeks, a walking track analysis with open field test (WTA), hystomorphometry (number of myelinated fibers (n), fiber density (FD) and fiber area (FA) and soleus and gastrocnemius muscle weight ratios (MWR) were evaluated. The Student t-test was used for statistical analysis. Results: At 4 and 12 weeks the Study Group had a significantly higher n and FD (p =.043 and.033). The SMWR was significantly higher in the Study Group at 12 weeks (p =.0207). Conclusions: Seeding the distal nerve suture with nerve fragments increases the number of myelinated fibers, the FD and the SMWR. The technique seems promising and deserves further investigation to clarify the mechanisms involved and its functional effects.
Original languageEnglish
Pages (from-to)27-35
Number of pages9
JournalActa Chirurgica Belgica
Volume118
Publication statusPublished - 2018

All Science Journal Classification (ASJC) codes

  • Surgery

Fingerprint Dive into the research topics of 'Seeding nerve sutures with minced nerve-graft (MINE-G): a simple method to improve nerve regeneration in rats'. Together they form a unique fingerprint.

  • Cite this