Relazione tra SNPs ai loci caseinici e tipizzazione lattoproteica nella capra Girgentana

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[automatically translated] The phylogeny of Girgentana race still remains more or less unknown, although the most viable hypothesis would seem that that relates to the goat Falconeri or Markor (Himalayas). The numerical strength of nell'ultimi years race has undergone a remarkable fall, so as to be numbered among the breeds in danger of extinction. Girgentana goat's milk was used only for direct consumption. When it entered into force on Regulation prohibiting the sale of milk to the detail, the zoo-economic value of Girgentana race has fallen sharply, contributing to the decrease in numbers of the breed in the last two decades. The revaluation of the race would be of fundamental importance, it has been found that the milk of the goat Girgentana presents rare protein variants, which could take a significant role in human nutrition. The greater presence of milk proteins are caseins alphaS1 (CSN1S1), beta (CSN2), alphaS2 (CSN1S2) and kappa (CSN3). In different goat breeds, the casein loci are highly polymorphic, and some genetic variants have an effect on milk production. The purpose of this study was to estimate the variability of casein loci in Girgentana race and determine whether there is a correlation between the dell'alphaS1-casein polymorphisms at the molecular level and isoelectric focusing (IEF), which determines the expression of protein itself. Although the IEF is a fast and inexpensive technique can not differentiate all the alleles at this locus. Alleles with the same isoelectric point (pI) overlap. You can, however, divide individuals into groups based on the content in alphaS1-casein: high content, medium and low in. They were genotyped 40 individuals (20 goats and 20 goats) for 9 SNPs and a deletion (exon 9) to CSN1S1 locus, 7 SNPs to CSN2 locus, 3 SNPs to CSN1S2 locus and 13 SNPs to CSN3 locus. The exon 9 genotypes were in agreement with the results all'IEF; individuals homozygous for the deletion have a very low level of S1-casein, heterozygous individuals have an intermediate level of protein expression, while individuals homozygous for the non-deletion show a complete epressione of the protein. For each gene haplotypes of genotyping they were constructed made by SNPs. They were found only a limited number of haplotypes within each locus. Six loci haplotypes for CSN1S1 and CSN2, 3 and 7 for CSN1S2 CSN3. The limited number of haplotypes demonstrates a close linkage disequilibrium (LD) between SNPs within each locus.
Original languageItalian
Number of pages1
Publication statusPublished - 2006

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