Pre-treatment with mesenchymal stem cells reduces ventilator-induced lung injury.

Maria Rosaria Bonsignore, Tomás Luque, Daniel Navajas, Laura Chimenti, Ramon Farre, José Ramírez

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31 Citations (Scopus)


ckground information: Bone marrow-derived mesenchymal stem cells (MSCs) reduce acute lung injury in animals challenged by bleomycin or bacterial lipopolysaccaride. It is not known, however, whether MSCs protect from ventilator- induced lung injury (VILI).Question of the study: Whether MSCs have a potential role in preventing or modulating VILI in healthy rats subjected to high-volume ventilation.Materials and methods: 24 Sprague-Dawley rats (250-300 g) were subjected to high- volume mechanical ventilation (25 ml/kg). MSCs (5x106) were intravenously or intratracheally administered (N=8 each) 30 min before starting over-ventilation and 8 rats were MSC-untreated. Spontaneously breathing anesthetised rats (N=8) served as controls. After 3 h of over-ventilation/control the animals were sacrificed and lung tissue and bronchoalveolar lavage fluid (BALF) were sampled for further analysis.Results: When compared with controls, MSC-untreated over-ventilated rats exhibited typical VILI features. Lung edema histological lung injury index, concentrations of total protein, interleukin-1β, macrophage inflammatory protein-2 and number of neutrophils in BALF and vascular cell adhesion protein-1 in lung tissue significantly increased in overventilated rats. All these indices of VILI moved significantly towards normalization in the rats treated with MSCs, whether intravenously or intratracheally.Answer to question: Both local and systemic pre-treatment with MSCs reduced VILI in a rat model.
Original languageEnglish
Pages (from-to)939-948
Number of pages10
JournalEuropean Respiratory Journal
Publication statusPublished - 2012

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine


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