Postnatal development of the dopaminergic signaling involved in the modulation of intestinal motility in mice

Flavia Mule', Mariangela Mastropaolo, Michelangelo Auteri, Rosa Maria Serio, Maria Grazia Zizzo, Gaetano Felice Caldara, Domenico Nuzzo, Giacomo Cavallaro, Ilaria Amodeo, Marta Di Carlo, Monica Fumagalli, Fabio Mosca, Domenico Nuzzo, Marta Di Carlo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background:Since antidopaminergic drugs are pharmacological agents employed in the management of gastrointestinal motor disorders at all ages, we investigated whether the enteric dopaminergic system may undergo developmental changes after birth.Methods:Intestinal mechanical activity was examined in vitro as changes in isometric tension.Results:In 2-d-old (P2) mice, dopamine induced a contractile effect, decreasing in intensity with age, replaced, at the weaning (day 20), by a relaxant response. Both responses were tetrodotoxin (TTX)-insensitive. In P2, dopaminergic contraction was inhibited by D1-like receptor antagonist and mimicked by D1-like receptor agonist. In 90-d-old (P90) mice, the relaxation was reduced by both D1- and D2-like receptor antagonists, and mimicked by D1- and D2-like receptor agonists. In P2, contraction was antagonized by phospholipase C inhibitor, while in P90 relaxation was antagonized by adenylyl cyclase inhibitor and potentiated by phospholipase C inhibitor. The presence of dopamine receptors was assessed by immunofluorescence. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed a significant increase in D1, D2, and D3 receptor expression in proximal intestine with the age.Conclusion:In mouse small intestine, the response to dopamine undergoes developmental changes shifting from contraction to relaxation at weaning, as the consequence of D2-like receptor recruitment and increased expression of D1 receptors.
Original languageEnglish
Pages (from-to)440-447
Number of pages8
JournalPediatric Research
Volume80
Publication statusPublished - 2016

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Gastrointestinal Motility
Type C Phospholipases
Weaning
Dopamine
Tetrodotoxin
Dopamine Receptors
Small Intestine
Intestines
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Parturition
Pharmacology
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health

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Postnatal development of the dopaminergic signaling involved in the modulation of intestinal motility in mice. / Mule', Flavia; Mastropaolo, Mariangela; Auteri, Michelangelo; Serio, Rosa Maria; Zizzo, Maria Grazia; Caldara, Gaetano Felice; Nuzzo, Domenico; Cavallaro, Giacomo; Amodeo, Ilaria; Di Carlo, Marta; Fumagalli, Monica; Mosca, Fabio; Nuzzo, Domenico; Di Carlo, Marta.

In: Pediatric Research, Vol. 80, 2016, p. 440-447.

Research output: Contribution to journalArticle

Mule', Flavia ; Mastropaolo, Mariangela ; Auteri, Michelangelo ; Serio, Rosa Maria ; Zizzo, Maria Grazia ; Caldara, Gaetano Felice ; Nuzzo, Domenico ; Cavallaro, Giacomo ; Amodeo, Ilaria ; Di Carlo, Marta ; Fumagalli, Monica ; Mosca, Fabio ; Nuzzo, Domenico ; Di Carlo, Marta. / Postnatal development of the dopaminergic signaling involved in the modulation of intestinal motility in mice. In: Pediatric Research. 2016 ; Vol. 80. pp. 440-447.
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abstract = "Background:Since antidopaminergic drugs are pharmacological agents employed in the management of gastrointestinal motor disorders at all ages, we investigated whether the enteric dopaminergic system may undergo developmental changes after birth.Methods:Intestinal mechanical activity was examined in vitro as changes in isometric tension.Results:In 2-d-old (P2) mice, dopamine induced a contractile effect, decreasing in intensity with age, replaced, at the weaning (day 20), by a relaxant response. Both responses were tetrodotoxin (TTX)-insensitive. In P2, dopaminergic contraction was inhibited by D1-like receptor antagonist and mimicked by D1-like receptor agonist. In 90-d-old (P90) mice, the relaxation was reduced by both D1- and D2-like receptor antagonists, and mimicked by D1- and D2-like receptor agonists. In P2, contraction was antagonized by phospholipase C inhibitor, while in P90 relaxation was antagonized by adenylyl cyclase inhibitor and potentiated by phospholipase C inhibitor. The presence of dopamine receptors was assessed by immunofluorescence. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed a significant increase in D1, D2, and D3 receptor expression in proximal intestine with the age.Conclusion:In mouse small intestine, the response to dopamine undergoes developmental changes shifting from contraction to relaxation at weaning, as the consequence of D2-like receptor recruitment and increased expression of D1 receptors.",
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AU - Mule', Flavia

AU - Mastropaolo, Mariangela

AU - Auteri, Michelangelo

AU - Serio, Rosa Maria

AU - Zizzo, Maria Grazia

AU - Caldara, Gaetano Felice

AU - Nuzzo, Domenico

AU - Cavallaro, Giacomo

AU - Amodeo, Ilaria

AU - Di Carlo, Marta

AU - Fumagalli, Monica

AU - Mosca, Fabio

AU - Nuzzo, Domenico

AU - Di Carlo, Marta

PY - 2016

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N2 - Background:Since antidopaminergic drugs are pharmacological agents employed in the management of gastrointestinal motor disorders at all ages, we investigated whether the enteric dopaminergic system may undergo developmental changes after birth.Methods:Intestinal mechanical activity was examined in vitro as changes in isometric tension.Results:In 2-d-old (P2) mice, dopamine induced a contractile effect, decreasing in intensity with age, replaced, at the weaning (day 20), by a relaxant response. Both responses were tetrodotoxin (TTX)-insensitive. In P2, dopaminergic contraction was inhibited by D1-like receptor antagonist and mimicked by D1-like receptor agonist. In 90-d-old (P90) mice, the relaxation was reduced by both D1- and D2-like receptor antagonists, and mimicked by D1- and D2-like receptor agonists. In P2, contraction was antagonized by phospholipase C inhibitor, while in P90 relaxation was antagonized by adenylyl cyclase inhibitor and potentiated by phospholipase C inhibitor. The presence of dopamine receptors was assessed by immunofluorescence. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed a significant increase in D1, D2, and D3 receptor expression in proximal intestine with the age.Conclusion:In mouse small intestine, the response to dopamine undergoes developmental changes shifting from contraction to relaxation at weaning, as the consequence of D2-like receptor recruitment and increased expression of D1 receptors.

AB - Background:Since antidopaminergic drugs are pharmacological agents employed in the management of gastrointestinal motor disorders at all ages, we investigated whether the enteric dopaminergic system may undergo developmental changes after birth.Methods:Intestinal mechanical activity was examined in vitro as changes in isometric tension.Results:In 2-d-old (P2) mice, dopamine induced a contractile effect, decreasing in intensity with age, replaced, at the weaning (day 20), by a relaxant response. Both responses were tetrodotoxin (TTX)-insensitive. In P2, dopaminergic contraction was inhibited by D1-like receptor antagonist and mimicked by D1-like receptor agonist. In 90-d-old (P90) mice, the relaxation was reduced by both D1- and D2-like receptor antagonists, and mimicked by D1- and D2-like receptor agonists. In P2, contraction was antagonized by phospholipase C inhibitor, while in P90 relaxation was antagonized by adenylyl cyclase inhibitor and potentiated by phospholipase C inhibitor. The presence of dopamine receptors was assessed by immunofluorescence. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed a significant increase in D1, D2, and D3 receptor expression in proximal intestine with the age.Conclusion:In mouse small intestine, the response to dopamine undergoes developmental changes shifting from contraction to relaxation at weaning, as the consequence of D2-like receptor recruitment and increased expression of D1 receptors.

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