Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization

Matteo Levantino, Henning Tidow, Stephan Niebling, Diana Monteiro, Inokentijs Josts, Yunyun Gao, Yunyun Gao, Matteo Levantino

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

This work demonstrates a new method for investigating time-resolved structural changes in protein conformation and oligomerization via photocage-initiated time-resolved X-ray solution scattering by observing the ATP-driven dimerization of the MsbA nucleotide-binding domain. Photocaged small molecules allow the observation of single-turnover reactions of non-naturally photoactivatable proteins. The kinetics of the reaction can be derived from changes in X-ray scattering associated with ATP-binding and subsequent dimerization. This method can be expanded to any small-molecule-driven protein reaction with conformational changes traceable by X-ray scattering where the small molecule can be photocaged.
Original languageEnglish
Pages (from-to)667-672
Number of pages6
JournalIUCrJ
Volume5
Publication statusPublished - 2018

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • Biochemistry
  • General Materials Science
  • Condensed Matter Physics

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