PHENOTYPIC PROFILING OF OSTEOTROPIC BREAST CANCER CELLS

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Abstract

One of the preferred locations of metastases from breast canceris the bone tissue. On the other hand, it should be recalled thatmammary tumors with equal clinical diagnosis have a differentcourse, and also different metastatic progression. Therefore, itwould be helpful to have appropriate markers of osteotropism totest on the surgical cancer tissues, in order to predict the possiblepropensity of the breast cancer to generate bone metastasesand to adequate the therapeutic plan.We previously reported1,2 on the setting-up of an in vitro modelfor the study of the osteotropic propensity of breast cancer cellsand the influences exerted by the bone microenvironment on thecancer cells phenotype.Viable bone fragments, deriving from surgery on traumaticlesions of young subjects were washed from bio-contaminantsunder sterile conditions and placed into cell culture capsuleswith the proper culture medium supplemented with foetal bovineserum, L-glutamine, L-ascorbic acid and antibiotics. Theexplants were kept for controlled timing in the humidified incubatorin order to promote the release of resident cells, and thenco-cultured with under-confluent breast cancer cells (SKBR3),until confluence. The bone fragments were then recovered,washed, placed again in culture dishes and monitored daily. Thecells released from the bone fragments were then collected andprocessed for immunological characterization and proteomicprofiling. The proteomic profiles of the cells seeded into the bonefragments were compared with the original cell culture, revealingan interesting differential proteomic pattern. The collectionof identified proteins on the maps has reached up today thenumber of 373. Differentially expressed proteins between boneseededcells and wild type cells were about 30%. Among the differentiallyexpressed proteins were several proteins belonging tothe cytoskeleton remodelling and proteins of the class of calcium-binding cluster. The relevance of these protein clusters is discussed.
Original languageEnglish
Pages11-11
Number of pages1
Publication statusPublished - 2015

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