Phenotypic and genotypic evaluation of slimeproduction by conventional and molecularmicrobiological techniques

Antonio Cascio, Renata Capicotto, Angela Quirino, Emanuele Focà, Rossana Puccio, Giorgio Settimo Barreca, Maria Carla Liberto, Angelo Giuseppe Lamberti, Giovanni Matera, Alfredo Focà, Antonio Cascio

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20 Citations (Scopus)

Abstract

Twenty-nine staphylococcal isolates from different clinical samples were tested forslime production: phenotypic characterization was carried out using Christensen test(tube test) and Congo red agar plate test (CRA plate test), while the presence andexpression of icaA and icaD genes were evaluated by real-time PCR. In 79.3% ofstudied strains there was a concordance between slime production and presenceof icaA and icaD genes, and between lack of slime production and absence of both oronly one of the tested genes. In four of five strains where positive phenotype was notassociated with the presence of ica genes, gene co-expression (evaluated by mRNAdetermination) was lacking, while in only a case out of five, there was the presenceof transcripts without phenotype. Our study, for the first time, introduces real-timePCR for the detection of both icaA and icaD genes and their mRNA and, furthermore,addresses the relationship between slime phenotype absence and mRNA expression,in clinical isolates of coagulase-negative staphylococci.
Original languageEnglish
Pages (from-to)522-528
Number of pages7
JournalMicrobiological Research
Volume164
Publication statusPublished - 2007

All Science Journal Classification (ASJC) codes

  • Microbiology

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