[automatically translated] Culture "in vitro" blastocyst was developed in the early '80s in some species of farmed animals (Camous, 1984) and only later applied to human embryos. the technical aim was to increase the percentage of plant in IVF cycles by reducing the number of embryos transferred. The cultivation of blastocyst allowed, in fact, to make the transfer of embryos with high evolutionary potential and in perfect synchronism with the uterine endometrium. Through the system of co-culture it was possible to support the embryonic development "in vitro" from the single cell stage to the blastocyst with the aid of cell monolayers consisting of the genital tract cells, mostly tubal epithelium. It was only in the early ' 90 that the use of co-culture technique is made use of extra-genital cells, such as epithelial cells of the kidney scimmmia, better known as VERO cells. The VERO cells had in common with the cells of the genital tract mesodermal origin and showed also significant advantages: easy commercial distribution, unlimited availability and security. In recent years the co-culture has been supplanted by the use of new sequential stage-specific soils, with a composition similar to that of physiological genital tract secretions and, undoubtedly, more practical and safe. The purpose of our study is to compare the two embryo "of in vitro culture systems" to evaluate its effectiveness in terms of percentage of blastocyst formation, implant and pregnancy.
|Publication status||Published - 2004|