Abstract

Duchenne muscular dystrophy (DMD) is characterized by progressive skeletal muscle weakness. We havepreviously shown that low-intensity endurance training prevented muscle damage (Frinchi et al, Int J Sports Med2014). Since the effects of low-intensity endurance training on the the diaphragm in the mdx mouse model areunknown, in the same animals we investigated C x39 protein levels (Western blotting) in homogenates of thediaphragm before and after training. Mdx and wild-type (WT) mice were randomly assigned to sedentary (mdx-S,n=17; WT-S, n=19) or trained (mdx-EX, n=14; WT-EX, n=16) groups. Low-intensity endurance training (running ona wheel) was done 5 days/week for 6 weeks at progressively increasing time (15 min to 1 h) and speed (rpm from16 to 24, distance covered during training sessions from 48 to 288 m). C ompared to our previous analysis ofskeletal muscles changes in gastrocnemius and quadriceps, showing decreased muscle damage in trained vssedentary mdx mice, analysis of protein level of C x39 showed similar values in diaphragm homogenates fromsedentary and trained mdx mice.These preliminary data suggest that prevention of muscle necrosis after mild training does not occur in thediaphragm. As a speculation, continuous work of diaphragm vs intermittent work of skeletal muscle might at leastpartly account for the different results obtained in respiratory and locomotor muscle.
Original languageEnglish
Number of pages1
Publication statusPublished - 2014

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