Neurons and ECM regulate occludin localization in brain endothelial cells

Gabriella Schiera, Libero Italo Giannola, Giovanni Savettieri, Viviana De Caro, Italia Di Liegro, Giulia Giandalia, Viviana De Caro, Libero Italo Giannola, Giovanni Savettieri, Alessandro Cestelli, Giovanna Laura Pitarresi, Giulia Giandalia, Stefania D'Agostino

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91 Citations (Scopus)


We report that extracellular matrix and neurons modulate the expression of occludin, one of the main components of tight junctions, by rat brain endothelial cells (RBE4.B). Of the three extracellular matrix proteins which we tested (collagen I, collagen IV, and laminin), collagen IV stimulated at the best the expression of occludin mRNA. The corresponding protein, however, was not synthesized. Significant amounts of occludin accumulated only when RBE4.B cells were cultured on collagen IV-coated inserts, in the presence of cortical neurons, plated on laminin-coated companion wells. Finally, occludin segregated at the cell periphery, only when endothelial cells were co- cultured with neurons for ≥ 1 week. (C) 2000 Lippincott Williams and Wilkins.
Original languageEnglish
Pages (from-to)1081-1084
Number of pages4
Publication statusPublished - 2000

All Science Journal Classification (ASJC) codes

  • General Neuroscience

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