Microvesicles shed by oligodendroglioma cells and rheumatoid synovial fibroblasts contain aggrecanase activity

Italia Di Liegro, Alessandra Lo Cicero, Iwona Majkowska, Hideaki Nagase, Hideaki Nagase, Hideaki Nagase, Linda Troeberg, Italia Di Liegro

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Membrane microvesicle shedding is an active process and occurs in viable cells with no signs of apoptosis or necrosis. We report here that microvesicles shed by oligodendroglioma cells contain an 'aggrecanase' activity, cleaving aggrecan at sites previously identified as targets for adamalysin metalloproteinases with disintegrin and thrombospondin domains (ADAMTSs). Degradation was inhibited by EDTA, the metalloproteinase inhibitor GM6001 and by tissue inhibitor of metalloproteinases (TIMP)-3, but not by TIMP-1 or TIMP-2. This inhibitor profile indicates that the shed microvesicles contain aggrecanolytic ADAMTS(s) or related TIMP-3-sensitive metalloproteinase(s). The oligodendroglioma cells were shown to express the three most active aggrecanases, namely Adamts1, Adamts4 and Adamts5, suggesting that one or more of these enzymes may be responsible for the microvesicle activity. Microvesicles shed by rheumatoid synovial fibroblasts similarly degraded aggrecan in a TIMP-3-sensitive manner. Our findings raise the novel possibility that microvesicles may assist oligodendroglioma and rheumatoid synovial fibroblasts to invade through aggrecan-rich extracellular matrices
Original languageEnglish
Pages (from-to)229-233
Number of pages5
JournalMatrix Biology
Volume31
Publication statusPublished - 2012

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Fingerprint Dive into the research topics of 'Microvesicles shed by oligodendroglioma cells and rheumatoid synovial fibroblasts contain aggrecanase activity'. Together they form a unique fingerprint.

Cite this