Mechanisms underlying hyperpolarization evoked by P2Y receptor activation in mouse distal colon

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In murine colonic circular muscle, ATP mediates fast component of the nerve-evoked inhibitory junction potentials, via activation of P2Yreceptors and opening of apamin-sensitive Ca2+-dependent K+ channels. We investigated, using microelectrode recordings, the intracellular eventsfollowing P2Y-receptor activation by electrical field stimulation or by adenosine 5′-O-2-thiodiphosphate (ADPβS), ATP stable analogue. The fastinhibitoryjunction potential amplitude was reduced by thapsigargin or ciclopiazonic acid (CPA), sarcoplasmic reticulum Ca2+-ATPase inhibitors,by ryanodine, which inhibits Ca2+ release from ryanodine-sensitive stores, and by 9-(tetrahydro-2-furanyl)-9H-purin-6-amine (SQ 22,536), anadenylyl cyclase inhibitor. Fast-inhibitory junction potentials were enhanced by 2-aminoethoxy-diphenylborate (2-APB), an IP3 receptor inhibitoror by {1-[6((17β-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl]-1H-pyrrole-2,5-dione} (U-73122), a phospholipase C inhibitor. ADPβSinduced hyperpolarization that was significantly reduced by apamin, thapsigargin, CPA, ryanodine, 2-APB and SQ 22,536, but it was not modifiedby U-73122. Forskolin, an adenylyl cyclase activator, induced hyperpolarization that was inhibited by SQ 22,536, apamin or ryanodine. Inconclusion, in murine colon, apamin-sensitive hyperpolarization induced by activation of P2Y receptors is mainly mediated by release of Ca2+from intracellular ryanodine-dependent stores via a mechanism involving adenylyl cyclase.
Original languageEnglish
Pages (from-to)174-180
Number of pages7
JournalEuropean Journal of Pharmacology
Publication statusPublished - 2006

All Science Journal Classification (ASJC) codes

  • Pharmacology

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