Mast cells crosstalk with B cells in the gut and sustain IgA response in the inflamed intestine

Beatrice Belmonte, Claudio Tripodo, Alessandro Gulino, Monika Adori, Francesca Mion, Shamila Vibhushan, Silvia Tonon, Viviana Valeri, Carlo E.M. Pucillo, Ulrich Blank, Gregory Gautier, Gunilla B. Karlsson Hedestam

Research output: Contribution to journalArticlepeer-review

Abstract

B lymphocytes are among the cell types whose effector functions are modulated by mast cells (MCs). The B/MC crosstalk emerged in several pathological settings, notably the colon of inflammatory bowel disease (IBD) patients is a privileged site in which MCs and IgA+ cells physically interact. Herein, by inducing conditional depletion of MCs in red MC and basophil (RMB) mice, we show that MCs control B cell distribution in the gut and IgA serum levels. Moreover, in dextran sulfate sodium (DSS)-treated RMB mice, the presence of MCs is fundamental for the enlargement of the IgA+ population in the bowel and the increase of systemic IgA production. Since both conventional B-2 and peritoneal-derived B cells populate the intestine and communicate with MCs in physiological conditions and during inflammation, we further explored this interplay through the use of co-cultures. We show that MCs finely regulate different aspects of splenic B cell biology while peritoneal B cells are unresponsive to the supporting effects provided by MCs. Interestingly, peritoneal B cells induce a pro-inflammatory skewing in MCs, characterized by increased ST2 and TNF-α expression. Altogether, this study uncovers the versatility of the B/MC liaison and highlights key aspects for the resolution of intestinal inflammation.
Original languageEnglish
Pages (from-to)445-458
Number of pages14
JournalEuropean Journal of Immunology
Volume51
Publication statusPublished - 2021

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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