L'Embriologia della Procreazione Medicalmente Assistita (PMA) in ambito forense

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Abstract

[automatically translated] The Embryology OF ASSISTED REPRODUCTION (PMA) IN SCOPE FORENSIC CARRA ELENA Department of Science and Technology Biological Chemical and Pharmaceutical, University of Palermo On Judicial Authority assignment is performed genetic analysis on human embryo degenerated and reconstruction of the path of the patients gametes subjected to PMA at 4 playback biology centers to highlight any significant irregularities in accordance with current legislation. This is the first commissioned surveys on the national territory they see engaged the forensic biologist at one of the border areas of reproductive medicine. With the pronouncement of the consultation in May 2009 the facility of L.nr.40 / 2004 has not changed. You can create embryos only for purposes of procreation and remain prohibitions to their cryopreservation, suppression and selection purposes eugenics. It was declared that the human embryo cryopreserved and thawed was irreversibly compromised in development: purpose of the investigation was to determine their amenability to married couple specification. By microscopy they were identified some particulates, so that the embryo's presence - inside of the straw - was questioned. Preliminarily, it made use of animal biological model to evaluate the extent to which the cellular degeneration level it could prevent the recognition. The results indicated that for the sea urchin eggs after 8 days was no longer the morphological recognition possible, while, for embryos some cell characteristics could also be appreciable to 20 days except that induced the lysis process. The case assumed aspects of particular importance, because it could not be excluded that the content of the straw had been made from oocyte degenerated (and not from embryo) and, consequently, was not detectable to microscopy; However, the demonstration of what -simple theoretically in line was thwarted by cellular degradation associated to the small DNA content (1 oocyte) representing the experimental condition suitable to the genesis of unstable allelic profiles. The method adopted for the recovery of the content of the straw (absorption on 3MM Whatman paper and subdivision of the sample into 7 parts to ensure experimental reproducibility), as well as, calibration of the kinetics of amplification for DNA Low Copy Number, contributed to the DNA typing and verifying that the observed particles were attributable to degenerate embryonic cellular matrix. The sample analysis of biological cards, medical records, IVF registers, etc .. united computerization of individual findings for the board type (data pick up, women, men, total oocytes, technical, mature oocytes, semen, embryos, oocytes discarded, discarded embryos, frozen eggs, frozen embryos, transferred embryos, doctor, biologist, analysis, notes) allowed the path reconstruction commissioned by highlighting the modus operandi of health in contravention of articles 13 and 14 L.nr. 40/2004 cited. He has contributed to the DNA typing and verifying that the observed particles were attributable to degenerate embryonic cellular matrix. The sample analysis of biological cards, medical records, IVF registers, etc .. united computerization of individual findings for the board type (data pick up, women, men, total oocytes, technical, mature oocytes, semen, embryos, oocytes discarded, discarded embryos, frozen eggs, frozen embryos, transferred embryos, doctor, biologist, analysis, notes) allowed the path reconstruction commissioned by highlighting the modus operandi of health in contravention of articles 13 and 14
Original languageItalian
Number of pages1
Publication statusPublished - 2013

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