Isolation and culture of colon cancer stem cells.

Sebastiano Bonventre, Giorgio Stassi, Matilde Todaro, Patrizia Cammareri, Maria Giovanna Francipane, Ylenia Lombardo

Research output: Contribution to journalArticlepeer-review

79 Citations (Scopus)

Abstract

Cancer stem cells (CSCs) resemble normal stem cells in several ways. Both cell types are self-renewing and when they divide, one of the daughter cells differentiates while the other retains stem cell properties, including the ability to divide in the same way again. CSCs have been demonstrated to exist in several solid tumors, including colon carcinoma; these cells are able to initiate and sustain tumor growth. There are essentially three different methods to isolate CSCs: establishment culture, the MACS (magnetic cell sorting) technology, and the FACS (fluorescence-activated cell sorting) technology.
Original languageEnglish
Pages (from-to)311-324
JournalMethods in Cell Biology
Volume86
Publication statusPublished - 2008

All Science Journal Classification (ASJC) codes

  • Cell Biology

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