Introduction of a novel pathway for IAA biosynthesis to rhizobia alters vech root nodule developmt

Giancarlo Moschetti, Esther Imperlini, Enza Lonardo, Bruno Campion, Serena Camerini, Bruno Campion, Beatrice Senatore, Giuseppe L. Rotino, Roberto Defez, Carmen Bianco

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)

Abstract

We introduced into Rhizobium leguminosarum bv. viciae LPR1105 a new pathway for the biosynthesis of the auxin, indole-3-acetic acid (IAA), under the control of a stationary phase-activated promoter active both in free-liv- ing bacteria and bacteroids. The newly introduced genes are the iaaM gene from Pseudomonas savastanoi and the tms2 gene from Agrobacterium tumefaciens. Free-living bacteria harbouring the promoter-iaaMtms2 construct release into the growth medium 14-fold more IAA than the wild-type parental strain. This IAA overproducing R. l. viciae, the RD20 strain, elicits the development of vetch root nodules containing up to 60-fold more IAA than nodules infected by the wild-type strain LPR1105. Vetch root nodules derived from RD20 are fewer in number per plant, heavier in terms of dry weight and show an enlarged and more active meri- stem. A signiWcant increase in acetylene reduction activity was measured in nodules elicited in vetch by RD20.
Original languageEnglish
Pages (from-to)67-77
Number of pages11
JournalArchives of Microbiology
Volume190
Publication statusPublished - 2008

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

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