Influence of metal ions on thermal aggregation of bovine serum albumin: Aggregation kinetics and structural changes

Maurizio Leone, Valeria Militello, Giovanna Navarra, Armida Torreggiani, Anna Tinti

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Abstract

Metal ions are implicated in protein aggregation processes of several neurodegenerative pathologies. In this work the effects of Cu(II) and Zn(II) ions on heat-induced structural modifications of bovine serum albumin (BSA) were studied, with the aim of delineating the role of these ions in the early stages of proteins aggregation kinetics. A joint application of different techniques was used. The aggregate growth was followed by dynamic light scattering measurements, whereas the conformational changes occurring in the protein structure were monitored by Raman and IR spectroscopy. Both in absence and in presence of metal ions, heating treatment gave rise to b-structures to the detriment of a-helix conformation of BSA. The temperature of protein unfolding was not sensitively affected by the presence of Zn(II) or Cu(II) ions; on the contrary, only Zn(II) ions slightly promoted the heat-induced aggregation of the protein, since bigger aggregates were formed in their presence. The different efficacy of the Cu(II) and Zn(II) ions in promoting the BSA aggregation were highlighted by Raman measurements, assessing the role of His residues in metal binding. A distinct polypeptide folding of the two metal–BSA systems takes place, since the predominant mode of metal binding depends on metal. In particular, in Zn–BSA the metal coordination involves the imidazole Ns atom of His which can promote inter-molecular cross-linking. 2009 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)1729-1738
Number of pages10
JournalJournal of Inorganic Biochemistry
Volume103
Publication statusPublished - 2009

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Bovine Serum Albumin
Metal ions
Agglomeration
Hot Temperature
Metals
Ions
Kinetics
Proteins
Pathology
Dynamic light scattering
Protein Unfolding
Raman spectroscopy
Conformations
Infrared spectroscopy
Raman Spectrum Analysis
Heating
Atoms
Peptides
Temperature
Growth

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Inorganic Chemistry

Cite this

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title = "Influence of metal ions on thermal aggregation of bovine serum albumin: Aggregation kinetics and structural changes",
abstract = "Metal ions are implicated in protein aggregation processes of several neurodegenerative pathologies. In this work the effects of Cu(II) and Zn(II) ions on heat-induced structural modifications of bovine serum albumin (BSA) were studied, with the aim of delineating the role of these ions in the early stages of proteins aggregation kinetics. A joint application of different techniques was used. The aggregate growth was followed by dynamic light scattering measurements, whereas the conformational changes occurring in the protein structure were monitored by Raman and IR spectroscopy. Both in absence and in presence of metal ions, heating treatment gave rise to b-structures to the detriment of a-helix conformation of BSA. The temperature of protein unfolding was not sensitively affected by the presence of Zn(II) or Cu(II) ions; on the contrary, only Zn(II) ions slightly promoted the heat-induced aggregation of the protein, since bigger aggregates were formed in their presence. The different efficacy of the Cu(II) and Zn(II) ions in promoting the BSA aggregation were highlighted by Raman measurements, assessing the role of His residues in metal binding. A distinct polypeptide folding of the two metal–BSA systems takes place, since the predominant mode of metal binding depends on metal. In particular, in Zn–BSA the metal coordination involves the imidazole Ns atom of His which can promote inter-molecular cross-linking. 2009 Elsevier Inc. All rights reserved.",
keywords = "Bovine Serum Albumin; Raman Spectroscopy, Infrared Spectroscopy; Thermal aggregation; Copper and Zinc ions",
author = "Maurizio Leone and Valeria Militello and Giovanna Navarra and Armida Torreggiani and Anna Tinti",
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language = "English",
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TY - JOUR

T1 - Influence of metal ions on thermal aggregation of bovine serum albumin: Aggregation kinetics and structural changes

AU - Leone, Maurizio

AU - Militello, Valeria

AU - Navarra, Giovanna

AU - Torreggiani, Armida

AU - Tinti, Anna

PY - 2009

Y1 - 2009

N2 - Metal ions are implicated in protein aggregation processes of several neurodegenerative pathologies. In this work the effects of Cu(II) and Zn(II) ions on heat-induced structural modifications of bovine serum albumin (BSA) were studied, with the aim of delineating the role of these ions in the early stages of proteins aggregation kinetics. A joint application of different techniques was used. The aggregate growth was followed by dynamic light scattering measurements, whereas the conformational changes occurring in the protein structure were monitored by Raman and IR spectroscopy. Both in absence and in presence of metal ions, heating treatment gave rise to b-structures to the detriment of a-helix conformation of BSA. The temperature of protein unfolding was not sensitively affected by the presence of Zn(II) or Cu(II) ions; on the contrary, only Zn(II) ions slightly promoted the heat-induced aggregation of the protein, since bigger aggregates were formed in their presence. The different efficacy of the Cu(II) and Zn(II) ions in promoting the BSA aggregation were highlighted by Raman measurements, assessing the role of His residues in metal binding. A distinct polypeptide folding of the two metal–BSA systems takes place, since the predominant mode of metal binding depends on metal. In particular, in Zn–BSA the metal coordination involves the imidazole Ns atom of His which can promote inter-molecular cross-linking. 2009 Elsevier Inc. All rights reserved.

AB - Metal ions are implicated in protein aggregation processes of several neurodegenerative pathologies. In this work the effects of Cu(II) and Zn(II) ions on heat-induced structural modifications of bovine serum albumin (BSA) were studied, with the aim of delineating the role of these ions in the early stages of proteins aggregation kinetics. A joint application of different techniques was used. The aggregate growth was followed by dynamic light scattering measurements, whereas the conformational changes occurring in the protein structure were monitored by Raman and IR spectroscopy. Both in absence and in presence of metal ions, heating treatment gave rise to b-structures to the detriment of a-helix conformation of BSA. The temperature of protein unfolding was not sensitively affected by the presence of Zn(II) or Cu(II) ions; on the contrary, only Zn(II) ions slightly promoted the heat-induced aggregation of the protein, since bigger aggregates were formed in their presence. The different efficacy of the Cu(II) and Zn(II) ions in promoting the BSA aggregation were highlighted by Raman measurements, assessing the role of His residues in metal binding. A distinct polypeptide folding of the two metal–BSA systems takes place, since the predominant mode of metal binding depends on metal. In particular, in Zn–BSA the metal coordination involves the imidazole Ns atom of His which can promote inter-molecular cross-linking. 2009 Elsevier Inc. All rights reserved.

KW - Bovine Serum Albumin; Raman Spectroscopy, Infrared Spectroscopy; Thermal aggregation; Copper and Zinc ions

UR - http://hdl.handle.net/10447/40921

M3 - Article

VL - 103

SP - 1729

EP - 1738

JO - Journal of Inorganic Biochemistry

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SN - 0162-0134

ER -