Inflamed adult pharynx tissues and swimming larva of Ciona intestinalis share CiTNFα-producing cells

Research output: Contribution to journalArticle

30 Citations (Scopus)


In situ hybridisation and mmunohistochemistryanalyses have shown that the Ciona intestinalis tumour necrosis factor alpha gene (CiTNFα), which has been previously cloned and sequenced, is expressed either during the inflammatory pharynx response to lipopolysaccharide (LPS) or during the swimming larval phase of development.Granulocytes with large granules and compartment/morula cells are CiTNFα-producing cells in both inflamed pharynxand larvae. Pharynx vessel endothelium also takes part in the inflammatory response. Haemocyte nodules in the vessel lumen or associated with the endothelium suggest theinvolvement of CiTNFα in recruiting lymphocyte-like cells and promoting the differentiation of inflammatory haemocytes.Specific antibodies against a CiTNFα peptide have identified a 43-kDa cell-bound form of the protein. Observations of pharynx histological sections (at 4 and 8 h post-LPSinoculation) from naive and medium-inoculated ascidians have confirmed the CiTNFα-positive tissue response. Larvalhistological sections and whole-mount preparations have revealed that CiTNFα is expressed by trunk mesenchyme,preoral lobe and tunic cells, indicating CiTNFα-expressing cell immigration events and an ontogenetic role.
Original languageEnglish
Pages (from-to)299-311
Number of pages13
JournalCell and Tissue Research
Publication statusPublished - 2010

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Fingerprint Dive into the research topics of 'Inflamed adult pharynx tissues and swimming larva of Ciona intestinalis share CiTNFα-producing cells'. Together they form a unique fingerprint.

  • Cite this