Identification of highly effective antitrypanosomal compounds in essential oils from the Apiaceae family

Maurizio Bruno, Riccardo Petrelli, Stefano Dall'Acqua, Loredana Cappellacci, Anders Hofer, Fariba Heshmati Afshar, Giovanni Benelli, Farahnaz Ranjbarian, Kevin Cianfaglione, Romilde Iannarelli, Filippo Maggi, Stephane L. Ngahang Kamte, Stefania Sut

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The Apiaceae family encompasses aromatic plants of economic importance employed in foodstuffs, beverages, perfumery, pharmaceuticals and cosmetics. Apiaceae are rich sources of essential oils because of the wealth of secretory structures (ducts and vittae) they are endowed with. The Apiaceae essential oils are available on an industrial level because of the wide cultivation and disposability of the bulky material from which they are extracted as well as their relatively cheap price. In the fight against protozoal infections, essential oils may represent new therapeutic options. In the present work, we focused on a panel of nine Apiaceae species (Siler montamon, Sison amomum, Echinophora spinosa, Kundmannia sicula, Crithmum maritimum, Helosciadium nodiforum, Pimpinella anisum, Heracleum sphondylium and Trachyspermum cunmi) and their essential oils as a model for the identification of trypanocidal compounds to be used as alternative/integrative therapies in the treatment of Human African trypanosomiasis (HAT) and as starting material for drug design. The evaluation of inhibitory effects of the Apiaceae essential oils against Trypanosoma brucei showed that some of them (E. spinosa, S. amomum, C. maritimwn and H. nodifloruin) were active, with EC50 in the range 2.7-10.7 mu g/mL. Most of these oils were selective against T. brucei, except the one from C. maritimum that was highly selective against the BALB/3T3 mammalian cells. Testing nine characteristic individual components (a-pinene, sabinene, alpha-phellandrene, p-cymene, limonene, beta-ocimene, gamma-terpinene, terpinolene, and myristicin) of these oils, we showed that some of them had much higher selectivity than the oils themselves. Terpinolene was particularly active with an EC50 value of 0.035 mu g/rnL (0.26 mu M) and a selectivity index (SI) of 180. Four other compounds with EC50 in the range 1.0-6.0 mu g/mL (7.4-44 mu M) had also good SI: a-pinene (> 100), beta-ocimene (> 91), limonene (> 18) and sabinene ( > 17). In conclusion, these results highlight that the essential oils from the Apiaceae family are a reservoir of substances to be used as leading compounds for the development of natural drugs for the treatment of HAT.
Original languageEnglish
Pages (from-to)154-165
Number of pages12
JournalEcotoxicology and Environmental Safety
Volume156
Publication statusPublished - 2018

All Science Journal Classification (ASJC) codes

  • Pollution
  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health

Cite this

Bruno, M., Petrelli, R., Dall'Acqua, S., Cappellacci, L., Hofer, A., Afshar, F. H., ... Sut, S. (2018). Identification of highly effective antitrypanosomal compounds in essential oils from the Apiaceae family. Ecotoxicology and Environmental Safety, 156, 154-165.

Identification of highly effective antitrypanosomal compounds in essential oils from the Apiaceae family. / Bruno, Maurizio; Petrelli, Riccardo; Dall'Acqua, Stefano; Cappellacci, Loredana; Hofer, Anders; Afshar, Fariba Heshmati; Benelli, Giovanni; Ranjbarian, Farahnaz; Cianfaglione, Kevin; Iannarelli, Romilde; Maggi, Filippo; Ngahang Kamte, Stephane L.; Sut, Stefania.

In: Ecotoxicology and Environmental Safety, Vol. 156, 2018, p. 154-165.

Research output: Contribution to journalArticle

Bruno, M, Petrelli, R, Dall'Acqua, S, Cappellacci, L, Hofer, A, Afshar, FH, Benelli, G, Ranjbarian, F, Cianfaglione, K, Iannarelli, R, Maggi, F, Ngahang Kamte, SL & Sut, S 2018, 'Identification of highly effective antitrypanosomal compounds in essential oils from the Apiaceae family', Ecotoxicology and Environmental Safety, vol. 156, pp. 154-165.
Bruno, Maurizio ; Petrelli, Riccardo ; Dall'Acqua, Stefano ; Cappellacci, Loredana ; Hofer, Anders ; Afshar, Fariba Heshmati ; Benelli, Giovanni ; Ranjbarian, Farahnaz ; Cianfaglione, Kevin ; Iannarelli, Romilde ; Maggi, Filippo ; Ngahang Kamte, Stephane L. ; Sut, Stefania. / Identification of highly effective antitrypanosomal compounds in essential oils from the Apiaceae family. In: Ecotoxicology and Environmental Safety. 2018 ; Vol. 156. pp. 154-165.
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author = "Maurizio Bruno and Riccardo Petrelli and Stefano Dall'Acqua and Loredana Cappellacci and Anders Hofer and Afshar, {Fariba Heshmati} and Giovanni Benelli and Farahnaz Ranjbarian and Kevin Cianfaglione and Romilde Iannarelli and Filippo Maggi and {Ngahang Kamte}, {Stephane L.} and Stefania Sut",
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T1 - Identification of highly effective antitrypanosomal compounds in essential oils from the Apiaceae family

AU - Bruno, Maurizio

AU - Petrelli, Riccardo

AU - Dall'Acqua, Stefano

AU - Cappellacci, Loredana

AU - Hofer, Anders

AU - Afshar, Fariba Heshmati

AU - Benelli, Giovanni

AU - Ranjbarian, Farahnaz

AU - Cianfaglione, Kevin

AU - Iannarelli, Romilde

AU - Maggi, Filippo

AU - Ngahang Kamte, Stephane L.

AU - Sut, Stefania

PY - 2018

Y1 - 2018

N2 - The Apiaceae family encompasses aromatic plants of economic importance employed in foodstuffs, beverages, perfumery, pharmaceuticals and cosmetics. Apiaceae are rich sources of essential oils because of the wealth of secretory structures (ducts and vittae) they are endowed with. The Apiaceae essential oils are available on an industrial level because of the wide cultivation and disposability of the bulky material from which they are extracted as well as their relatively cheap price. In the fight against protozoal infections, essential oils may represent new therapeutic options. In the present work, we focused on a panel of nine Apiaceae species (Siler montamon, Sison amomum, Echinophora spinosa, Kundmannia sicula, Crithmum maritimum, Helosciadium nodiforum, Pimpinella anisum, Heracleum sphondylium and Trachyspermum cunmi) and their essential oils as a model for the identification of trypanocidal compounds to be used as alternative/integrative therapies in the treatment of Human African trypanosomiasis (HAT) and as starting material for drug design. The evaluation of inhibitory effects of the Apiaceae essential oils against Trypanosoma brucei showed that some of them (E. spinosa, S. amomum, C. maritimwn and H. nodifloruin) were active, with EC50 in the range 2.7-10.7 mu g/mL. Most of these oils were selective against T. brucei, except the one from C. maritimum that was highly selective against the BALB/3T3 mammalian cells. Testing nine characteristic individual components (a-pinene, sabinene, alpha-phellandrene, p-cymene, limonene, beta-ocimene, gamma-terpinene, terpinolene, and myristicin) of these oils, we showed that some of them had much higher selectivity than the oils themselves. Terpinolene was particularly active with an EC50 value of 0.035 mu g/rnL (0.26 mu M) and a selectivity index (SI) of 180. Four other compounds with EC50 in the range 1.0-6.0 mu g/mL (7.4-44 mu M) had also good SI: a-pinene (> 100), beta-ocimene (> 91), limonene (> 18) and sabinene ( > 17). In conclusion, these results highlight that the essential oils from the Apiaceae family are a reservoir of substances to be used as leading compounds for the development of natural drugs for the treatment of HAT.

AB - The Apiaceae family encompasses aromatic plants of economic importance employed in foodstuffs, beverages, perfumery, pharmaceuticals and cosmetics. Apiaceae are rich sources of essential oils because of the wealth of secretory structures (ducts and vittae) they are endowed with. The Apiaceae essential oils are available on an industrial level because of the wide cultivation and disposability of the bulky material from which they are extracted as well as their relatively cheap price. In the fight against protozoal infections, essential oils may represent new therapeutic options. In the present work, we focused on a panel of nine Apiaceae species (Siler montamon, Sison amomum, Echinophora spinosa, Kundmannia sicula, Crithmum maritimum, Helosciadium nodiforum, Pimpinella anisum, Heracleum sphondylium and Trachyspermum cunmi) and their essential oils as a model for the identification of trypanocidal compounds to be used as alternative/integrative therapies in the treatment of Human African trypanosomiasis (HAT) and as starting material for drug design. The evaluation of inhibitory effects of the Apiaceae essential oils against Trypanosoma brucei showed that some of them (E. spinosa, S. amomum, C. maritimwn and H. nodifloruin) were active, with EC50 in the range 2.7-10.7 mu g/mL. Most of these oils were selective against T. brucei, except the one from C. maritimum that was highly selective against the BALB/3T3 mammalian cells. Testing nine characteristic individual components (a-pinene, sabinene, alpha-phellandrene, p-cymene, limonene, beta-ocimene, gamma-terpinene, terpinolene, and myristicin) of these oils, we showed that some of them had much higher selectivity than the oils themselves. Terpinolene was particularly active with an EC50 value of 0.035 mu g/rnL (0.26 mu M) and a selectivity index (SI) of 180. Four other compounds with EC50 in the range 1.0-6.0 mu g/mL (7.4-44 mu M) had also good SI: a-pinene (> 100), beta-ocimene (> 91), limonene (> 18) and sabinene ( > 17). In conclusion, these results highlight that the essential oils from the Apiaceae family are a reservoir of substances to be used as leading compounds for the development of natural drugs for the treatment of HAT.

KW - Apiaceae; BALB/3T3; Essential oils; Human African trypanosomiasis; Trypanosoma brucei; 3T3 Cells; Alkenes; Animals; Apiaceae; Benzyl Compounds; Cyclohexenes; Dioxolanes; Inhibitory Concentration 50; Mice; Monoterpenes; Oils, Volatile; Plant Oils; Pyrogall

UR - http://hdl.handle.net/10447/300632

M3 - Article

VL - 156

SP - 154

EP - 165

JO - Ecotoxicology and Environmental Safety

JF - Ecotoxicology and Environmental Safety

SN - 0147-6513

ER -