Hydrocarbonoclastic bacteria from a polluted harbour in Sicily (Italy): isolation, identification and characterization of their biotechnological potential.

Research output: Contribution to conferenceOtherpeer-review

Abstract

Metabolicspecializationisageneralbiologicalprinciplethatshapestheassemblyofmicrobialcommunities.Hydrocarbonoclasticbacteria(HCB)aremarinebacteriathatarespecialisedinhydrocarbondegradationandusehydrocarbonsalmostexclusivelyasuniqueCandenergysource.HCBbecomedominantinoil-‐impactedenvironmentsandareparticularlyadaptedforremovalofhydrocarbonsfromcontaminatedsitesthroughbioremediationtreatments.ThepetrochemicalsiteofPriolo-‐Augusta-‐Melilli(Sicily,Italy),isaSiteofNationalInterest(SIN)duetohighlevelsofenvironmentalcontaminationofthecoastlineandaspecific"nationalprogramofenvironmentalremediationandrestoration"wasdevelopedinordertoallowremediationandrestorationofcontaminatedsites.Inordertotoidentifythekeyhydrocarbondegradersandexplorethenaturalbioremediationpotentialofthecontaminatedarea,atotalofsixsedimentandseawatercoreswerecollectedinsidethePrioloHarbour(SR,Italy).Afterbiological(bacterialpopulationdiversity,PCR-‐DGGE)andchemical-‐physicalcharacterisation(quali-‐,quantitativemeasuresofhydrocarbonsandheavymetals)sampleswereused,separately,tosetenrichmentculturesonmineralbrothcontainingdifferentmixturesoflinear(C16,C18,C20)andaromatic(phenatrene,pyrene,biphenylanddibenzothiophene)hydrocarbonsandCrudeOil(ArabianLigthCrudeOil).Atotalof159isolates(82fromseawaterand77fromsediments)wereobtained.Thesequencingof16SrDNAshowedthemostoftheisolatesbelongedto,Alcanivorax(44%),Marinobacter(17%)andOleibacter(9%)genera.Dataobtainedfromassaysofbiodegradationrevealedasmoreinterestingbacteriapresentaveryhighpotentialofapplicationinbioremediationtechniques;furthermoredataobtainedgiveadeeperunderstandingofthebiochemicalcausesofmetabolicspecializationandcouldserveasafoundationforthefieldofsyntheticecology,wheretheobjectivewouldbetorationallyengineertheassemblyofamicrobialcommunitytoperformadesiredbiotransformation.
Original languageEnglish
Pages331-331
Number of pages1
Publication statusPublished - 2013

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