Human papillomaviruses (HPVs) are agents of the most common sexually transmitted diseases. Both in menand in women, HPVs cause a variety of clinical symptoms ranging from warts to cancer. It has been shownthat infection with oncogenic HPV is the major cause of development of cervical cancer in women and lessfrequently of penile cancers in men. HPV infects epithelial cells and can bind other cell types. Its presence insemen is widely known, but the effects on fertility and reproductive function are still controversial.Partners of women with high-grade squamous intraepithelial lesions were enrolled. From eachsubject seminal parameters were evaluated. HPV was detected and genotyped both in total semen and inswim up-fraction.In order to verify the cause-effect relationship between HPV presence in semen and sperm quality, wedeveloped a new approach (differential cell lysis, DNA extraction and nested PCR) to evaluate viruslocalization in the different semen components. Our data indicate a correlation between HPV infection ofexfoliated cells and reduction of sperm cell motility. Moreover, we analyzed the physical state of HPV18DNA in sperm cells by qPCR. Our results suggest a possible integration event during which a disruption ofthe HPV genome occurs, causing the deletion of E1 and E2 genes. The data support the proposed role ofHPV in decreased male and couple fertility and prompt new possible consequences of the viral infection insemen.
|Number of pages||1|
|Publication status||Published - 2016|