Granzyme A as a potential biomarker of Mycobacterium tuberculosis infection and disease

Nadia Rosalia Caccamo, Giuliana Guggino, Francesco Dieli, Marco Pio La Manna, Diana Di Liberto, Marco P. La Manna, Giuliana Guggino, Valentina Orlando, Valentina Vanini, Elisa Petruccioli, Stella Cutrera, Francesco Dieli, Diana Di Liberto, Nadia Caccamo, Delia Goletti, Stella Cutrera, Valentina Orlando

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Cytotoxic molecules such as granulysin, perforin and granzymes produced by cytolytic T cells directly contribute to immune defense against tuberculosis (TB). In search for novel TB biomarkers, we have evaluated the levels of granzyme A in plasma obtained from QuantiFERON-TB Gold In tube (QFT-IT) assays from patients with active TB disease and subjects with latent TB infection (LTBI).Granzyme A serum levels in TB patients were significantly lower than values found in LTBI subjects even after subtraction of the unstimulated levels from the antigen-stimulated responses. The receiver operator characteristics (ROC) curve analysis comparing TB patients and LTBI groups, showed that at a cut-off value of granzyme A of <3.425. pg/ml, the sensitivity and the specificity of the assay were 29.41% and 94.74%, respectively.Our results suggest that granzyme A could be considered another biomarker of TB, that can be used, other than IFN-γ, to discriminate between patients with active TB and LTBI subjects in a well characterized cohort of confirmed Mycobacterium tuberculosis-infected individuals.
Original languageEnglish
Pages (from-to)87-91
Number of pages5
JournalImmunology Letters
Volume166
Publication statusPublished - 2015

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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