GEOBACTER METALLIREDUCENS MUTANTS FOR CHROMIUM BIOREMEDIATION

Chicca, I; Marsili, E

    Research output: Other contribution

    Abstract

    Geobacter metallireducens is known to use oxidized metals such as Fe(III) and Mn(IV) as terminal extracellular electron acceptors. G. metallireducens can reduce the highly soluble and toxic Cr(VI) to its insoluble form Cr(III), thus enabling chromium removal from contaminated water and soil. This process is limited by the low toxicity resistance of G. metallireducens to Cr(VI). Genome shuffling can generate mutants with improved phenotype. In our work we are carrying out G. metallireducens genome shuffling, in order to increase its resistance to Cr(VI). In Geobacter species, metal toxicity resistance is correlated to the respiration rate, hence to the reduction rate of the metallic electron acceptor, when soluble electron acceptors are depleted. Our results show that mutants obtained are more resistant than wild type strain to Cr(VI). We characterized the metal reduction process of G. metallireducens wild type and mutants in potentiostat-controlled electrochemical cells through microbial biofilm voltammetry
    Original languageEnglish
    Publication statusPublished - 2011

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    Bioremediation
    Chromium
    Metals
    Toxicity
    Electrons
    Genes
    Electrochemical cells
    Poisons
    Biofilms
    Voltammetry
    Soils
    chromium hexavalent ion
    Water

    Cite this

    GEOBACTER METALLIREDUCENS MUTANTS FOR CHROMIUM BIOREMEDIATION. / Chicca, I; Marsili, E.

    2011, .

    Research output: Other contribution

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    abstract = "Geobacter metallireducens is known to use oxidized metals such as Fe(III) and Mn(IV) as terminal extracellular electron acceptors. G. metallireducens can reduce the highly soluble and toxic Cr(VI) to its insoluble form Cr(III), thus enabling chromium removal from contaminated water and soil. This process is limited by the low toxicity resistance of G. metallireducens to Cr(VI). Genome shuffling can generate mutants with improved phenotype. In our work we are carrying out G. metallireducens genome shuffling, in order to increase its resistance to Cr(VI). In Geobacter species, metal toxicity resistance is correlated to the respiration rate, hence to the reduction rate of the metallic electron acceptor, when soluble electron acceptors are depleted. Our results show that mutants obtained are more resistant than wild type strain to Cr(VI). We characterized the metal reduction process of G. metallireducens wild type and mutants in potentiostat-controlled electrochemical cells through microbial biofilm voltammetry",
    keywords = "Bioremediation; Genome shuffling; Chromium",
    author = "{Chicca, I; Marsili, E} and Gabriele Pastorella",
    year = "2011",
    language = "English",
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    T1 - GEOBACTER METALLIREDUCENS MUTANTS FOR CHROMIUM BIOREMEDIATION

    AU - Chicca, I; Marsili, E

    AU - Pastorella, Gabriele

    PY - 2011

    Y1 - 2011

    N2 - Geobacter metallireducens is known to use oxidized metals such as Fe(III) and Mn(IV) as terminal extracellular electron acceptors. G. metallireducens can reduce the highly soluble and toxic Cr(VI) to its insoluble form Cr(III), thus enabling chromium removal from contaminated water and soil. This process is limited by the low toxicity resistance of G. metallireducens to Cr(VI). Genome shuffling can generate mutants with improved phenotype. In our work we are carrying out G. metallireducens genome shuffling, in order to increase its resistance to Cr(VI). In Geobacter species, metal toxicity resistance is correlated to the respiration rate, hence to the reduction rate of the metallic electron acceptor, when soluble electron acceptors are depleted. Our results show that mutants obtained are more resistant than wild type strain to Cr(VI). We characterized the metal reduction process of G. metallireducens wild type and mutants in potentiostat-controlled electrochemical cells through microbial biofilm voltammetry

    AB - Geobacter metallireducens is known to use oxidized metals such as Fe(III) and Mn(IV) as terminal extracellular electron acceptors. G. metallireducens can reduce the highly soluble and toxic Cr(VI) to its insoluble form Cr(III), thus enabling chromium removal from contaminated water and soil. This process is limited by the low toxicity resistance of G. metallireducens to Cr(VI). Genome shuffling can generate mutants with improved phenotype. In our work we are carrying out G. metallireducens genome shuffling, in order to increase its resistance to Cr(VI). In Geobacter species, metal toxicity resistance is correlated to the respiration rate, hence to the reduction rate of the metallic electron acceptor, when soluble electron acceptors are depleted. Our results show that mutants obtained are more resistant than wild type strain to Cr(VI). We characterized the metal reduction process of G. metallireducens wild type and mutants in potentiostat-controlled electrochemical cells through microbial biofilm voltammetry

    KW - Bioremediation; Genome shuffling; Chromium

    UR - http://hdl.handle.net/10447/64980

    M3 - Other contribution

    ER -