FTIR analysis of the high pressure response of native insulin assemblies

Federica Piccirilli, Mangialardo, Stefano Lupi, Federica Piccirilli, Andrea Perucchi, Postorino

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

It is widely recognized that a central role in conferring stability to the structure of proteins against misfolding and aggregation is played by the formation of oligomers. The case of insulin is prototypical in this respect: in our body it is stored up in stable inactive hexameric assemblies whereas only in its monomeric form it recovers the role of regulating carbohydrate and fat metabolism. In the present paper, exploiting the optimal coupling between FTIR spectroscopy and diamond anvil cell technique, we probe the stability of different insulin oligomeric forms under high pressure, namely over the ranges 0–15 kbar for water solution and 0–80 kbar for dry powder. Results obtained show different responses to volume compression for the different assemblies being the structure of monomers and dimers remarkably more affected by compression than hexamers. Moreover by comparing the results obtained using water solution and dry powder we were able to draw important considerations about the role of water in the high pressure unfolding processes.
Original languageEnglish
Pages (from-to)159-165
Number of pages7
JournalJournal of Molecular Structure
Volume1050
Publication statusPublished - 2013

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Piccirilli, F., Mangialardo, Lupi, S., Piccirilli, F., Perucchi, A., & Postorino (2013). FTIR analysis of the high pressure response of native insulin assemblies. Journal of Molecular Structure, 1050, 159-165.