Floral scent in a sexually deceptive Ophrys orchid: from headspace collections to solvent extractions

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Abstract

Sexually deceptive orchid flowers use visual, tactile and olfactory cues of female insects in order to attract males of one or a few closely related species as pollinators. Ophrys L. is the most species-rich genus of sexually deceptive orchids in the Mediterranean Basin. Despite Ophrys pollinated by Andrena male bees use alkanes and mainly alkenes with specific double-bond positions as key signals that trigger pseudocopulatory behavior, some volatile organic compounds (VOCs) with low molecular weight were found as long-range attractants non-eliciting copulatory behavior. Since floral scents in Ophrys have been extensively studied by solvent extractions here we aimed to understand which floral volatiles are found when two different collection methods are used in Ophrys panormitana flowers. By knowing their chemical composition, we could better understand the scent chemistry of this Ophrys species without overlooking VOCs which could also have a function in its pollination biology.Scent samples collected by dynamic headspace and by solvent extraction were analyzed by gas chromatography/mass spectrometry (GC/MS).The floral scent of O. panormitana is composed by a bouquet of VOCs with lower and higher molecular weights. The headspace samples contained VOCs with higher volatility (mainly one aliphatic alcohol and two aliphatic ketones) whereas the solvent extracts were composed by VOCs with lower volatility (exclusively long-chain alkanes and alkenes). Overlapping in VOCs between headspace and solvent samples were not found. For the first time Andrena nigroaenea was observed during the pseudocopulation and removing the pollinaria of a flower of O. panormitana.
Original languageEnglish
Pages (from-to)00-
Number of pages6
JournalPLANT SIGNALING & BEHAVIOR
Volume14
Publication statusPublished - 2019

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Ophrys
volatile organic compounds
headspace analysis
odors
Andrena
alkenes
flowers
alkanes
molecular weight
pollinating insects
attractants
ketones
Orchidaceae
sampling
Apoidea
pollination
chemistry
alcohols
chemical composition
basins

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

@article{e98dc3bcc5e74c5e964f54df86fcc2cf,
title = "Floral scent in a sexually deceptive Ophrys orchid: from headspace collections to solvent extractions",
abstract = "Sexually deceptive orchid flowers use visual, tactile and olfactory cues of female insects in order to attract males of one or a few closely related species as pollinators. Ophrys L. is the most species-rich genus of sexually deceptive orchids in the Mediterranean Basin. Despite Ophrys pollinated by Andrena male bees use alkanes and mainly alkenes with specific double-bond positions as key signals that trigger pseudocopulatory behavior, some volatile organic compounds (VOCs) with low molecular weight were found as long-range attractants non-eliciting copulatory behavior. Since floral scents in Ophrys have been extensively studied by solvent extractions here we aimed to understand which floral volatiles are found when two different collection methods are used in Ophrys panormitana flowers. By knowing their chemical composition, we could better understand the scent chemistry of this Ophrys species without overlooking VOCs which could also have a function in its pollination biology.Scent samples collected by dynamic headspace and by solvent extraction were analyzed by gas chromatography/mass spectrometry (GC/MS).The floral scent of O. panormitana is composed by a bouquet of VOCs with lower and higher molecular weights. The headspace samples contained VOCs with higher volatility (mainly one aliphatic alcohol and two aliphatic ketones) whereas the solvent extracts were composed by VOCs with lower volatility (exclusively long-chain alkanes and alkenes). Overlapping in VOCs between headspace and solvent samples were not found. For the first time Andrena nigroaenea was observed during the pseudocopulation and removing the pollinaria of a flower of O. panormitana.",
author = "Sergio Rosselli and Pietro Zito and Maggio, {Antonella Maria} and Maurizio Sajeva and Maurizio Bruno",
year = "2019",
language = "English",
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journal = "PLANT SIGNALING & BEHAVIOR",
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TY - JOUR

T1 - Floral scent in a sexually deceptive Ophrys orchid: from headspace collections to solvent extractions

AU - Rosselli, Sergio

AU - Zito, Pietro

AU - Maggio, Antonella Maria

AU - Sajeva, Maurizio

AU - Bruno, Maurizio

PY - 2019

Y1 - 2019

N2 - Sexually deceptive orchid flowers use visual, tactile and olfactory cues of female insects in order to attract males of one or a few closely related species as pollinators. Ophrys L. is the most species-rich genus of sexually deceptive orchids in the Mediterranean Basin. Despite Ophrys pollinated by Andrena male bees use alkanes and mainly alkenes with specific double-bond positions as key signals that trigger pseudocopulatory behavior, some volatile organic compounds (VOCs) with low molecular weight were found as long-range attractants non-eliciting copulatory behavior. Since floral scents in Ophrys have been extensively studied by solvent extractions here we aimed to understand which floral volatiles are found when two different collection methods are used in Ophrys panormitana flowers. By knowing their chemical composition, we could better understand the scent chemistry of this Ophrys species without overlooking VOCs which could also have a function in its pollination biology.Scent samples collected by dynamic headspace and by solvent extraction were analyzed by gas chromatography/mass spectrometry (GC/MS).The floral scent of O. panormitana is composed by a bouquet of VOCs with lower and higher molecular weights. The headspace samples contained VOCs with higher volatility (mainly one aliphatic alcohol and two aliphatic ketones) whereas the solvent extracts were composed by VOCs with lower volatility (exclusively long-chain alkanes and alkenes). Overlapping in VOCs between headspace and solvent samples were not found. For the first time Andrena nigroaenea was observed during the pseudocopulation and removing the pollinaria of a flower of O. panormitana.

AB - Sexually deceptive orchid flowers use visual, tactile and olfactory cues of female insects in order to attract males of one or a few closely related species as pollinators. Ophrys L. is the most species-rich genus of sexually deceptive orchids in the Mediterranean Basin. Despite Ophrys pollinated by Andrena male bees use alkanes and mainly alkenes with specific double-bond positions as key signals that trigger pseudocopulatory behavior, some volatile organic compounds (VOCs) with low molecular weight were found as long-range attractants non-eliciting copulatory behavior. Since floral scents in Ophrys have been extensively studied by solvent extractions here we aimed to understand which floral volatiles are found when two different collection methods are used in Ophrys panormitana flowers. By knowing their chemical composition, we could better understand the scent chemistry of this Ophrys species without overlooking VOCs which could also have a function in its pollination biology.Scent samples collected by dynamic headspace and by solvent extraction were analyzed by gas chromatography/mass spectrometry (GC/MS).The floral scent of O. panormitana is composed by a bouquet of VOCs with lower and higher molecular weights. The headspace samples contained VOCs with higher volatility (mainly one aliphatic alcohol and two aliphatic ketones) whereas the solvent extracts were composed by VOCs with lower volatility (exclusively long-chain alkanes and alkenes). Overlapping in VOCs between headspace and solvent samples were not found. For the first time Andrena nigroaenea was observed during the pseudocopulation and removing the pollinaria of a flower of O. panormitana.

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