Abstract
| Original language | English |
|---|---|
| Number of pages | 0 |
| Journal | Journal of Cellular Physiology |
| Publication status | Published - 2017 |
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All Science Journal Classification (ASJC) codes
- Physiology
- Clinical Biochemistry
- Cell Biology
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Extracellular Hsp70 Enhances Mesoangioblast Migration via an Autocrine Signaling Pathway. / Sconzo, Gabriella; Geraci, Fabiana; Spinello, Walter; Barreca, Maria Magdalena; Cavalieri, Vincenzo; Tinnirello, Rosaria; Turturici, Giuseppina; Tinnirello, Rosaria; Kaur, Punit; Geraci, Fabiana; Asea, Alexzander A. A.
In: Journal of Cellular Physiology, 2017.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Extracellular Hsp70 Enhances Mesoangioblast Migration via an Autocrine Signaling Pathway
AU - Sconzo, Gabriella
AU - Geraci, Fabiana
AU - Spinello, Walter
AU - Barreca, Maria Magdalena
AU - Cavalieri, Vincenzo
AU - Tinnirello, Rosaria
AU - Turturici, Giuseppina
AU - Tinnirello, Rosaria
AU - Kaur, Punit
AU - Geraci, Fabiana
AU - Asea, Alexzander A. A.
PY - 2017
Y1 - 2017
N2 - Mouse mesoangioblasts are vessel-associated progenitor stem cells endowed with the ability of multipotent mesoderm differentiation. Therefore, they represent a promising tool in the regeneration of injured tissues. Several studies have demonstrated that homing of mesoangioblasts into blood and injured tissues are mainly controlled by cytokines/chemokines and other inflammatory factors. However, little is known about the molecular mechanisms regulating their ability to traverse the extracellular matrix (ECM). Here, we demonstrate that membrane vesicles released by mesoangioblasts contain Hsp70, and that the released Hsp70 is able to interact by an autocrine mechanism with Toll-like receptor 4 (TLR4) and CD91 to stimulate migration. We further demonstrate that Hsp70 has a positive role in regulating matrix metalloproteinase 2 (MMP2) and MMP9 expression and that MMP2 has a more pronounced effect on cell migration, as compared to MMP9. In addition, the analysis of the intracellular pathways implicated in Hsp70 regulated signal transduction showed the involvement of both PI3K/AKT and NF-κB. Taken together, our findings present a paradigm shift in our understanding of the molecular mechanisms that regulate mesoangioblast stem cells ability to traverse the extracellular matrix (ECM).
AB - Mouse mesoangioblasts are vessel-associated progenitor stem cells endowed with the ability of multipotent mesoderm differentiation. Therefore, they represent a promising tool in the regeneration of injured tissues. Several studies have demonstrated that homing of mesoangioblasts into blood and injured tissues are mainly controlled by cytokines/chemokines and other inflammatory factors. However, little is known about the molecular mechanisms regulating their ability to traverse the extracellular matrix (ECM). Here, we demonstrate that membrane vesicles released by mesoangioblasts contain Hsp70, and that the released Hsp70 is able to interact by an autocrine mechanism with Toll-like receptor 4 (TLR4) and CD91 to stimulate migration. We further demonstrate that Hsp70 has a positive role in regulating matrix metalloproteinase 2 (MMP2) and MMP9 expression and that MMP2 has a more pronounced effect on cell migration, as compared to MMP9. In addition, the analysis of the intracellular pathways implicated in Hsp70 regulated signal transduction showed the involvement of both PI3K/AKT and NF-κB. Taken together, our findings present a paradigm shift in our understanding of the molecular mechanisms that regulate mesoangioblast stem cells ability to traverse the extracellular matrix (ECM).
UR - http://hdl.handle.net/10447/215873
M3 - Article
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
SN - 0021-9541
ER -