Expression in Streptomyces lividans of Nonomuraea genes cloned in an artificial chromosome

Rosa Alduina, Giuseppe Gallo, Anna Giardina, Anna Maria Puglia, Clelia Ferraro, Stefano Donadio, Andrea Scaloni, Giovanni Renzone, Alba Contino

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

A bacterial artificial chromosomal library of Nonomuraea sp. ATCC39727 was constructed using Escherichia coli-Streptomyces artificial chromosome (ESAC) and screened for the presence of dbv genes known to be involved in the biosynthesis of the glycopeptide A40926. dbv genes were cloned as two large, partially overlapping, fragments and transferred into the host Streptomyces lividans, thus generating strains S. lividansColon, two colonsNmESAC50 and S. lividansColon, two colonsNmESAC57. The heterologous expression of Nonomuraea genes in S. lividans was successfully demonstrated by using combined RT-PCR and proteomic approaches. MALDI-TOF analysis revealed that a Nonomuraea ABC transporter is expressed as two isoforms in S. lividans. Moreover, its expression may not require a Nonomuraea positive regulator at all, as it is present at similar levels in both clones even though S. lividansColon, two colonsNmESAC57 lacks regulatory genes. Considered together, these results show that S. lividans expresses Nonomuraea genes from their own promoters and support the idea that S. lividans can be a good host for genetic analysis of Nonomuraea.
Original languageEnglish
Pages (from-to)656-662
Number of pages7
JournalApplied Microbiology and Biotechnology
Volume68
Publication statusPublished - 2005

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Microbiology
  • Microbiology (medical)
  • Biotechnology
  • Applied Microbiology and Biotechnology

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