TY - JOUR
T1 - Evaluation of mechanical and morphologic features of PLLA membranes as supports for perfusion cells culture systems
AU - Montesanto, Salvatore
AU - Brucato, Valerio Maria Bartolo
AU - La Carrubba, Vincenzo
AU - La Carrubba, null
PY - 2016
Y1 - 2016
N2 - Porous biodegradable PLLA membranes, which can be used as supports for perfusion cell culture systems were designed, developed and characterized. PLLA membranes were prepared via diffusion induced phase separation (DIPS). A glass slab was coated with a binary PLLA–dioxane solution (8 wt.% PLLA) via dip coating, then pool immersed in two subsequent coagulation baths, and finally dried in a humidity-controlled environment. Surface and mechanical properties were evaluated by measuring pore size, porosity via scanning electron microscopy, storage modulus, loss modulus and loss angle by using a dynamic mechanical analysis (DMA). Cell adhesion assays on different membrane surfaces were also performed by using a standard count method. Results provide new insights into the foaming methods for producing polymeric membranes and supply indications on how to optimise the fabrication parameters to design membranes for tissue cultures and regeneration.
AB - Porous biodegradable PLLA membranes, which can be used as supports for perfusion cell culture systems were designed, developed and characterized. PLLA membranes were prepared via diffusion induced phase separation (DIPS). A glass slab was coated with a binary PLLA–dioxane solution (8 wt.% PLLA) via dip coating, then pool immersed in two subsequent coagulation baths, and finally dried in a humidity-controlled environment. Surface and mechanical properties were evaluated by measuring pore size, porosity via scanning electron microscopy, storage modulus, loss modulus and loss angle by using a dynamic mechanical analysis (DMA). Cell adhesion assays on different membrane surfaces were also performed by using a standard count method. Results provide new insights into the foaming methods for producing polymeric membranes and supply indications on how to optimise the fabrication parameters to design membranes for tissue cultures and regeneration.
KW - Cell adhesion
KW - Condensed Matter Physics
KW - Materials Science (all)
KW - Mechanical Engineering
KW - Mechanical properties
KW - Mechanics of Materials
KW - PLLA membranes
KW - Cell adhesion
KW - Condensed Matter Physics
KW - Materials Science (all)
KW - Mechanical Engineering
KW - Mechanical properties
KW - Mechanics of Materials
KW - PLLA membranes
UR - http://hdl.handle.net/10447/202199
M3 - Article
VL - 69
SP - 841
EP - 849
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
ER -