Encapsulation of in vitro-derived propagules of two genotypes of Capparis spinosa (L.) from Pantelleria Island

Maria Germana', Nicolò Iacuzzi, Germana, Ozudogru

Research output: Contribution to journalArticlepeer-review

Abstract

Encapsulation technology, combining the advantages of zygotic or gamic seed with those of micropropagation, has recently attracted the interest of researchers as a new propagation approach, mainly due to the unsatisfying results of the traditional propagation strategies. The encapsulation of uninodal microcuttings (3-4 mm long) from two Sicilian Capparis spinosa (L.) genotypes (from Pantelleria Island: Tracino and Scauri) was evaluated, observing the influence of the calcium alginate coating and of three different growth regulators (PGRs): 6-Benzylaminopurine (BAP), Meta-Topolin (MT) and Zeatin (ZEA), on viability, regrowth and conversion of the propagules.Caper microcuttings were dissected and placed in different Murashige and Skoog-based artificial endosperms, enriched of MT or ZEA or BAP. The synthetic seeds obtained were sown on a medium with full strength salts concentration, enriched with 0,4 mg/L of naphtalene acetic acid and 0,7 mg/L of gibelleric acid. After 60 days, the following parameters were detected: viability, regrowth, number and length of the shoots and roots, conversion.The results confirm that encapsulation did not negatively affect the viability, which showed the highest percentage with BAP (100%) in Tracino and with ZEA (100%) in Scauri. Similar results were obtained in regrowth, with statistically significant differences among the three PGRs tested: Tracino showed the best regrowth on capsuled enriched with BAP (100%), Scauri with ZEA (100%). In addition, the synseed conversion was greatly affected by the PGR, and it was higher in artificial endosperm added with BAP in Tracino (56.6%) and ZEA in Scauri (23.3%) genotype.
Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalACTA HORTICULTURAE
Volume1285
Publication statusPublished - 2020

All Science Journal Classification (ASJC) codes

  • Horticulture

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