Electron transfer mechanism in Shewanella loihica PV- 4 biofilms formed atgraphite electrode

Gabriele Pastorella, Enrico Marsili, Xiaoming Zhang, Niamh Barry, Gabriele Pastorella, Jack O. Connolly, Anand Jain, Xiaoming Zhang, Robert Woolley, Satheesh Krishnamurthy

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    Abstract

    Electron transfer mechanisms in Shewanella loihica PV- 4 viable biofilms formed at graphiteelectrodes were investigated in potentiostat-controlled electrochemical cells poised atoxidative potentials (0.2 V vs. Ag/AgCl). Chronoamperometry (CA) showed a repeatablebiofilm growth of S. loihica PV- 4 on graphite electrode. CA, cyclic voltammetry (CV) andits first derivative shows that both direct electron transfer (DET) mediated electron transfer(MET) mechanism contributes to the overall anodic (oxidation) current. The maximumanodic current density recorded on graphite was 90 μA cm-2. Fluorescence emission spectrashows increased concentration of quinone derivatives and riboflavin in the cell-freesupernatant as the biofilm grows. Differential pulse voltammetry (DPV) show accumulationof riboflavin at the graphite interface, with the increase in incubation period. This is the firststudy to observe a gradual shift from DET to MET mechanism in viable S. loihica PV- 4biofilms.
    Original languageEnglish
    Pages (from-to)28-32
    Number of pages5
    JournalBioelectrochemistry
    Volume87
    Publication statusPublished - 2012

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Physical and Theoretical Chemistry
    • Electrochemistry

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    Pastorella, G., Marsili, E., Zhang, X., Barry, N., Pastorella, G., Connolly, J. O., Jain, A., Zhang, X., Woolley, R., & Krishnamurthy, S. (2012). Electron transfer mechanism in Shewanella loihica PV- 4 biofilms formed atgraphite electrode. Bioelectrochemistry, 87, 28-32.