Effects of cadmium exposure on sea urchin developmentassessed by SSH and RT-qPCR: metallothionein genesand their differential induction

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Abstract

In order to study the defense strategies activated by Paracentrotus lividus embryos in response to sublethaldoses of CdCl2, we compared the induced transcripts to that of control embryos by suppression subtractivehybridization technique. We isolated five metallothionein (MT) cDNAs and other genes related to detoxification, to signaling pathway components, to oxidative, reductive and conjugative biotransformation, to RNA maturation and protein synthesis. RT-qPCR analysis revealed that two of the five P. lividus MT (PlMT7 and PlMT8) genes appeared to be constitutively expressed and upregulated following cadmium treatment, whereas the other three genes (PlMT4, PlMT5, PlMT6) are specifically switched-on in response to cadmium treatment. Moreover, we found that this transcriptional induction is concentration dependent and that the cadmium concentration threshold for the gene activation is distinct for every gene. RT-qPCR experiments showed in fact that, among induced genes, PlMT5 gene is activated at a very low cadmium concentration (0.1 lM) whereas PlMT4 and PlMT6 are activated at intermediate doses (1–10 lM). Differently, PlMT7 and PlMT8 genes increase significantly their expression only in embryos treated with the highest dose (100 lM CdCl2). We found also that, in response to a lethal dose of cadmium (1 lM), only PlMT5 and PlMT6 mRNA levels increased further.These data suggest a hierarchical and orchestrated response of the P. lividus embryo to overcome differential environmental stressors that could interfere with a normaldevelopment.
Original languageEnglish
Pages (from-to)2157-2167
Number of pages11
JournalMolecular Biology Reports
Volume40
Publication statusPublished - 2013

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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