Effects in cigarette smoke stimulated bronchial epithelial cells of a corticosteroid entrapped into nanostructured lipid carriers

Gennara Cavallaro, Chiara Botto, Gaetano Giammona, Serena Di Vincenzo, Maria Luisa Bondì, Stefania Gerbino, Maria Ferraro, Serena Di Vincenzo, Mark Gjomarkaj, Elisabetta Pace

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

BackgroundNanomedicine studies have showed a great potential for drug delivery into the lung. In this manuscript nanostructured lipid carriers (NLC) containing Fluticasone propionate (FP) were prepared and their biocompatibility and effects in a human bronchial epithelial cell line (16-HBE) stimulated with cigarette smoke extracts (CSE) were tested.ResultsBiocompatibility studies showed that the NLC did not induce cell necrosis or apoptosis. Moreover, it was confirmed that CSE increased intracellular ROS production and TLR4 expression in bronchial epithelial cells and that FP-loaded NLC were more effective than free drug in modulating these processes. Finally, the nanoparticles increased GSH levels improving cell protection against oxidative stress.ConclusionsThe present study shows that NLC may be considered a promising strategy to improve corticosteroid mediated effects in cellular models associated to corticosteroid resistance. The NLC containing FP can be considered good systems for dosage forms useful for increasing the effectiveness of fluticasone decreasing its side effects
Original languageEnglish
Pages (from-to)46-60
Number of pages15
JournalJournal of Nanobiotechnology
Volume12
Publication statusPublished - 2014

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Pharmaceutical Science

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