Effect of single-species and mixed-species leaf leachate on bacterial communities in biofilms

Luigi Naselli Flores, Lan Wu, Christopher B. Blackwood, Laura G. Leff

Research output: Book/ReportOther report

12 Citations (Scopus)

Abstract

Dissolved organic matter in the form of leaf leachate represents an important carbon andenergy source in many lotic ecosystems. In this study, we investigated utilization of mono-specific and mixed-species leaf leachate and impacts on biofilm bacterial community structure. Ceramic tiles were incubated in a Northeast Ohio stream to allow for biofilm development and then exposed in the laboratory to glucose or leachate from: sugar maple (Acer saccharum), pin oak (Quercus palustris), maple oak, American beech (Fagus grandifolia), witchhazel (Hamamelis virginiana), or beech witchhazel. Bacterial responses to these amendments were compared to un-amended controls based on fluorescent in situ hybridization (FISH) targeting selected taxaand terminal restriction fragment length polymorphism (T-RFLP) of bacterial 16S rRNA genes; also changes in DOC concentrations were quantified. Generally, there were limited differences among communities as a result of leachate amendment, although specific taxa monitored by FISH exhibiteddifferential responses. There was no evidence that mixing of leachate from different leaf species created an effect different than what could be expected basedon monospecific experiments. Witch hazel solicited the greatest response, based on T-RFLP data, regardless of whether the community was exposed to witch hazel alone or witch hazel ? beech, accounting for 19% of the variability in Jaccard distance P\0.05) and 27% of the variability in Hellinger distance among profiles. In conclusion, we found that leaf leachate can be readily degraded but only in some cases did differences in leaf leachate among treespecies cause an alteration in community structure. Mixing of leachate from different leaf species did have an impact on DOC loss but did not alter community structure. The occurrence of particular compounds, such as those in witch hazel, may alter community structure suggesting that the presence and abundance of specific plant taxa can impact bacterial communities.
Original languageEnglish
PublisherSpringer
Number of pages12
ISBN (Print)0018-8158
Publication statusPublished - 2009

Publication series

NameHydrobiologia

Fingerprint

leachates
Hamamelis virginiana
bacterial communities
biofilm
leachate
leaves
community structure
Quercus palustris
Fagus grandifolia
fluorescence in situ hybridization
Fagus
restriction fragment length polymorphism
polymorphism
lotic systems
Acer saccharum
Acer saccharum subsp. saccharum
effect
ceramics
tiles
Acer

All Science Journal Classification (ASJC) codes

  • Aquatic Science

Cite this

Naselli Flores, L., Wu, L., Blackwood, C. B., & Leff, L. G. (2009). Effect of single-species and mixed-species leaf leachate on bacterial communities in biofilms. (Hydrobiologia). Springer.

Effect of single-species and mixed-species leaf leachate on bacterial communities in biofilms. / Naselli Flores, Luigi; Wu, Lan; Blackwood, Christopher B.; Leff, Laura G.

Springer, 2009. 12 p. (Hydrobiologia).

Research output: Book/ReportOther report

Naselli Flores, Luigi ; Wu, Lan ; Blackwood, Christopher B. ; Leff, Laura G. / Effect of single-species and mixed-species leaf leachate on bacterial communities in biofilms. Springer, 2009. 12 p. (Hydrobiologia).
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abstract = "Dissolved organic matter in the form of leaf leachate represents an important carbon andenergy source in many lotic ecosystems. In this study, we investigated utilization of mono-specific and mixed-species leaf leachate and impacts on biofilm bacterial community structure. Ceramic tiles were incubated in a Northeast Ohio stream to allow for biofilm development and then exposed in the laboratory to glucose or leachate from: sugar maple (Acer saccharum), pin oak (Quercus palustris), maple oak, American beech (Fagus grandifolia), witchhazel (Hamamelis virginiana), or beech witchhazel. Bacterial responses to these amendments were compared to un-amended controls based on fluorescent in situ hybridization (FISH) targeting selected taxaand terminal restriction fragment length polymorphism (T-RFLP) of bacterial 16S rRNA genes; also changes in DOC concentrations were quantified. Generally, there were limited differences among communities as a result of leachate amendment, although specific taxa monitored by FISH exhibiteddifferential responses. There was no evidence that mixing of leachate from different leaf species created an effect different than what could be expected basedon monospecific experiments. Witch hazel solicited the greatest response, based on T-RFLP data, regardless of whether the community was exposed to witch hazel alone or witch hazel ? beech, accounting for 19{\%} of the variability in Jaccard distance P\0.05) and 27{\%} of the variability in Hellinger distance among profiles. In conclusion, we found that leaf leachate can be readily degraded but only in some cases did differences in leaf leachate among treespecies cause an alteration in community structure. Mixing of leachate from different leaf species did have an impact on DOC loss but did not alter community structure. The occurrence of particular compounds, such as those in witch hazel, may alter community structure suggesting that the presence and abundance of specific plant taxa can impact bacterial communities.",
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N2 - Dissolved organic matter in the form of leaf leachate represents an important carbon andenergy source in many lotic ecosystems. In this study, we investigated utilization of mono-specific and mixed-species leaf leachate and impacts on biofilm bacterial community structure. Ceramic tiles were incubated in a Northeast Ohio stream to allow for biofilm development and then exposed in the laboratory to glucose or leachate from: sugar maple (Acer saccharum), pin oak (Quercus palustris), maple oak, American beech (Fagus grandifolia), witchhazel (Hamamelis virginiana), or beech witchhazel. Bacterial responses to these amendments were compared to un-amended controls based on fluorescent in situ hybridization (FISH) targeting selected taxaand terminal restriction fragment length polymorphism (T-RFLP) of bacterial 16S rRNA genes; also changes in DOC concentrations were quantified. Generally, there were limited differences among communities as a result of leachate amendment, although specific taxa monitored by FISH exhibiteddifferential responses. There was no evidence that mixing of leachate from different leaf species created an effect different than what could be expected basedon monospecific experiments. Witch hazel solicited the greatest response, based on T-RFLP data, regardless of whether the community was exposed to witch hazel alone or witch hazel ? beech, accounting for 19% of the variability in Jaccard distance P\0.05) and 27% of the variability in Hellinger distance among profiles. In conclusion, we found that leaf leachate can be readily degraded but only in some cases did differences in leaf leachate among treespecies cause an alteration in community structure. Mixing of leachate from different leaf species did have an impact on DOC loss but did not alter community structure. The occurrence of particular compounds, such as those in witch hazel, may alter community structure suggesting that the presence and abundance of specific plant taxa can impact bacterial communities.

AB - Dissolved organic matter in the form of leaf leachate represents an important carbon andenergy source in many lotic ecosystems. In this study, we investigated utilization of mono-specific and mixed-species leaf leachate and impacts on biofilm bacterial community structure. Ceramic tiles were incubated in a Northeast Ohio stream to allow for biofilm development and then exposed in the laboratory to glucose or leachate from: sugar maple (Acer saccharum), pin oak (Quercus palustris), maple oak, American beech (Fagus grandifolia), witchhazel (Hamamelis virginiana), or beech witchhazel. Bacterial responses to these amendments were compared to un-amended controls based on fluorescent in situ hybridization (FISH) targeting selected taxaand terminal restriction fragment length polymorphism (T-RFLP) of bacterial 16S rRNA genes; also changes in DOC concentrations were quantified. Generally, there were limited differences among communities as a result of leachate amendment, although specific taxa monitored by FISH exhibiteddifferential responses. There was no evidence that mixing of leachate from different leaf species created an effect different than what could be expected basedon monospecific experiments. Witch hazel solicited the greatest response, based on T-RFLP data, regardless of whether the community was exposed to witch hazel alone or witch hazel ? beech, accounting for 19% of the variability in Jaccard distance P\0.05) and 27% of the variability in Hellinger distance among profiles. In conclusion, we found that leaf leachate can be readily degraded but only in some cases did differences in leaf leachate among treespecies cause an alteration in community structure. Mixing of leachate from different leaf species did have an impact on DOC loss but did not alter community structure. The occurrence of particular compounds, such as those in witch hazel, may alter community structure suggesting that the presence and abundance of specific plant taxa can impact bacterial communities.

KW - Bacteria

KW - Biofilm

KW - In situ hybridization

KW - Leaf leachate

KW - Terminal restriction fragment length polymorphism

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BT - Effect of single-species and mixed-species leaf leachate on bacterial communities in biofilms

PB - Springer

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