Dynamics of hyaluronan aqueous solutions as assessed by fast field cycling NMR relaxometry

Giuseppe Alonzo, Pellegrino Conte, Alena Průšová, Jiří Kučerík

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Fast field cycling (FFC) NMR relaxometry has been used to study the conformational properties of aqueous solutions of hyaluronan (HYA) at three concentrations in the range 10 to 25 mg mL–1. Results revealed that, irrespective of the solution concentration, three different hydration layers surround hyaluronan. The inner layer consists of water molecules strongly retained in the proximity of the HYA surface. Because of their strong interactions with HYA, water molecules in this inner hydration layer are subject to very slow dynamics and have the largest correlation times. The other two hydration layers are made of water molecules which are located progressively further from the HYA surface. As a result, decreasing correlation times caused by faster molecular motion were measured. The NMRD profiles obtained by FFC-NMR relaxometry also showed peaks attributable to 1H–14N quadrupole interactions. Changes in intensity and position of the quadrupolar peaks in the NMRD profiles suggested that with increasing concentration the amido group is progressively involved in the formation of weak and transient intramolecular water bridging adjacent hyaluronan chains. In this work, FFC-NMR was used for the first time to obtain deeper insight into HYA–water interactions and proved itself a powerful and promising tool in hyaluronan chemistry.
Original languageEnglish
Pages (from-to)3023-3028
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume397
Publication statusPublished - 2010

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

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