Development and validation of a reliable method for studying the distribution pattern for opiates metabolites in brain

Paolo Procaccianti, Antonina Argo, Katia Guerrini, Daria Catalano, Cristina Borroni, Antonella Argo, Paolo Procaccianti, Lucia Dell'Acqua, Gabriella Roda, Veniero Gambaro, Fiorenza Farè

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Brain distribution pattern of “street” heroin metabolites (morphine and codeine) was investigated intwo fatalities due to “acute narcotism”. A suitable sample pretreatment prior to solid-phase-extractionwas developed to achieve a good recovery of the analytes and to eliminate the interfering species. Afterderivatization with MSTFA, samples were analyzed by GC/MS. Specificity, accuracy, precision and linearityof the method were evaluated; LOD and LOQ were, respectively, 10 ng/25 ng for morphine and 5 ng/10 ngfor codeine.This method was applied to the analysis of six brain areas (hippocampus, frontal lobe, occipital lobe, nuclei, bulb and pons) coming from two cases of heroin-related deaths. No evidence of accumulation of metabolites in a specific brain region was found
Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume73
Publication statusPublished - 2012

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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