Cultivable microorganisms associated with honeys of different geographical and botanical origin

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In this study, the composition of the cultivable microbial populations of 38 nectar honey and honeydewhoney samples of different botanical and geographical origin were assessed. After growth in specificmedia, various colonies with different appearance were isolated and purified before phenotypic(morphological, physiological and biochemical traits) and genotypic [randomly amplified polymorphicDNA (RAPD), repetitive DNA elements-PCR (rep-PCR) and restriction fragment length polymorphism(RFLP)] differentiation. The identification was carried out by 16S rRNA gene sequencing for bacteria and,in addition to RFLP, by sequencing the D1/D2 region of the 26S rRNA gene for yeasts and the 5.8S-ITSrRNA region for filamentous fungi. The results showed the presence of 13 species of bacteria, 5 of yeastsand 17 of filamentous fungi; the species most frequently isolated were Bacillus amyloliquefaciens,Zygosaccharomyces mellis and Aspergillus niger for the three microbial groups, respectively. The highestmicrobial diversity was found in multifloral honeys. No correlation among the microbial species and thebotanical/geographical origin was found, but some strains were highly adapted to these matrices sincethey were found in several samples of different origin.
Original languageEnglish
Pages (from-to)284-294
Number of pages11
JournalFood Microbiology
Publication statusPublished - 2014

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology


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