Control of Growth and Persistence of Listeria monocytogenes and beta-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings

Domenico Schillaci, Maria Grazia Cusimano, Vita Di Stefano, Maria Vitale, Maria La Giglia, Vincenzo Di Marco Lo Presti, Vincenzo Di Marco Lo Presti, Francesco Pomilio, Domenico Schillaci, Vita Di Stefano, Maria Grazia Cusimano, Maria Elisa La Giglia

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The main objective of this study was to evaluate the efficacy of thymol in controlling environmental contamination in food processing facilities. The effect of thymol was tested as an agent to prevent planktonic and bacterial biofilm growth of twenty-five Listeria monocytogenes isolates from a variety of foods and five Escherichia coli isolates from a farm. The E. coli isolates were positive for extended spectrum beta-lactamase (ESBL) genes. All isolates and reference strains were susceptible to thymol at Minimum inhibitory concentration (MIC) values ranging from 250 to 800 microg/mL. An interesting activity of interference with biofilm formation of L. monocytogenes and E. coli was found for thymol at sub-MIC concentrations of 200, 100, 75, and 50 microg/mL. Anti-biofilm activity ranging from 59.71% to 66.90% against pre-formed 24-h-old L. monocytogenes biofilms at concentrations of 500 or 800 microg/mL, corresponding to 2x MIC, was determined against free-living forms of six isolates chosen as the best or moderate biofilm producers among the tested strains. The property of thymol to attack L. monocytogenes biofilm formation was also observed at a concentration of 100 microg/mL, corresponding to 1/4 MIC, by using a stainless-steel model to simulate the surfaces in food industries. This study gives information on the use of thymol in food processing setting.
Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalMolecules
Volume25
Publication statusPublished - 2020

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Fingerprint

Dive into the research topics of 'Control of Growth and Persistence of Listeria monocytogenes and beta-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings'. Together they form a unique fingerprint.

Cite this