In the present paper we applied confocal microscopy and fluorescence technologies for studying the distribution and the oxidative activity of sea urchin (Paracentrotus lividus) mitochondria during development, by in vivo incubating eggs and embryos with cell-permeant MitoTracker probes. We calculated, by a mathematical model, the intensity values, the variations of intensity, and the variation index of incorporated fluorochromes. Data demonstrate that mitochondrial mass does not change during development, whereas mitochondrial respiration increases. In addition, starting from 16 blastomeres stage, some regions of the embryo contain organelles more active in oxygen consumption.
|Number of pages||9|
|Journal||Journal of Microscopy|
|Publication status||Published - 2007|
All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
Roccheri, M. C., Di Liegro, C. M., Spagnolo, F., Rinaldi, A. M., Morici, G., & Agnello, M. (2007). Confocal microscopy study of the distribution, content and activity of mitochondria during Paracentrotus lividus development. Journal of Microscopy, 228, 165-173.