Complete sequence, genotyping and comparative analysis of Pepino mosaic virus discovered isolates from Italy

Salvatore Davino, Mario Davino, Tiberini, Davino, Tomassoli

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Pepino mosaic virus (PepMV) causes economic damageto the tomato industry worldwide. In Italy, the virusis endemic in Sicily, an important tomato-production region,and a new outbreak has been recorded in Sardiniaafter the first PepMV appearance in 2001. Italian PepMVisolates from these two geographically distant regionswere molecularly characterized and the complete nucleotidesequence of four isolates was determined bywalking primer strategy. The complete nucleotide anddeduced amino acid sequences were analyzed and comparedwith published PepMV sequences. Italian isolatesbelong to the PepMV-Ch2 strain and had 97.2% to98.5% identity with those of the reference PepMV-Ch2deposited in GenBank. The two Sicilian isolates wereslightly more variable in the RdRp and TGB1 genes.This study provides new insights into the occurrenceand variability of PepMV isolates in Italy.
Original languageEnglish
Pages (from-to)437-442
Number of pages6
JournalJournal of Plant Pathology
Publication statusPublished - 2011


All Science Journal Classification (ASJC) codes

  • Plant Science

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