Comparison between adenosine triphosphate bioluminescence and aerobic colony count to assess surface sanitation in the hospital environment

Alberto Firenze, Omar Enzo Santangelo, Daniele Domenico Raia, Debora Piazza, Lucia Cannova, Luigi Aprea, Sandro Provenzano, Valentina Bonanno

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Adenosine triphosphate bioluminescence produced by the firefly luciferase has been successfully introduced to verify cleaning procedures in the food industry according to the Hazard Analysis Critical Control Point program. Our aim was to evaluate the reliability of bioluminescence as a tool to monitor the effectiveness of sanitation in healthcare settings, in comparison with the microbiological gold standard. Methods: 614 surfaces of various material were randomly sampled in Policlinico University Hospital units in Palermo, Italy, to detect adenosine triphosphate bioluminescence and aerobic colony count. Linear regression model and Pearson correlation coefficient were used to estimate the relationship between the two variables of the study. Results: Aerobic colony count median was 1.71 colony forming units/cm2 (interquartile range = 3.8), whereas adenosine triphosphate median was 59.9 relative light units/cm2 (interquartile range = 128.3). Pearson coefficient R2 was 0.09. Sensitivity and specificity of bioluminescence test with respect to microbiology were 46% and 71%, whereas positive predictive value and negative predictive value were 53% and 65%, respectively. Conclusion: According to our results, there seemed to be no linear correlation between aerobic colony count and adenosine triphosphate values, suggesting that current bioluminescence technology has not any proportional relationships with culturable microbes contaminating environmental surfaces in health-care settings.
Original languageEnglish
Pages (from-to)12710-12714
Number of pages5
JournalEpidemiology Biostatistics and Public Health
Volume15
Publication statusPublished - 2018

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Health Policy
  • Community and Home Care
  • Public Health, Environmental and Occupational Health

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