Combination of multiplex PCR and PCR-DGGE for monitoring common sourdough-associated Lactobacillus species

Luca Settanni; Sara Valmorri; Antonello Paparella; Luca Settanni; Aldo Corsetti; Douwe Van Sinderen; Giovanna Suzzi

Combination of multiplex PCR and PCR-DGGE for monitoring common sourdough-associated Lactobacillus species

Luca Settanni, Sara Valmorri, Antonello Paparella, Luca Settanni, Aldo Corsetti, Douwe Van Sinderen, Giovanna Suzzi

Scienze Agrarie, Alimentari e Forestali

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.

Original language	English
Pages (from-to)	3793-3796
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	72
Publication status	Published - 2006

All Science Journal Classification (ASJC) codes

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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Combination of multiplex PCR and PCR-DGGE for monitoring common sourdough-associated Lactobacillus species. / Settanni, Luca; Valmorri, Sara; Paparella, Antonello; Settanni, Luca; Corsetti, Aldo; Van Sinderen, Douwe; Suzzi, Giovanna.

In: Applied and Environmental Microbiology, Vol. 72, 2006, p. 3793-3796.

Research output: Contribution to journal › Article › peer-review

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abstract = "A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.",

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AU - Corsetti, Aldo

AU - Van Sinderen, Douwe

AU - Suzzi, Giovanna

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KW - PCR multiplex

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