Combination of multiplex PCR and PCR-DGGE for monitoring common sourdough-associated Lactobacillus species

Luca Settanni, Sara Valmorri, Antonello Paparella, Luca Settanni, Aldo Corsetti, Douwe Van Sinderen, Giovanna Suzzi

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.
Original languageEnglish
Pages (from-to)3793-3796
Number of pages4
JournalApplied and Environmental Microbiology
Volume72
Publication statusPublished - 2006

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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