The selective modulation of immunity is an emerging concept driven by the vast advances in our understandingof this crucial host defense system. Invertebrates have raised researchers’ interest as potential sources of newbioactive molecules owing to their antibacterial, anticancer and immunomodulatory activities. ALipoPolySaccharide (LPS) challenge in the ascidian Ciona intestinalis generates the transcript, Ci8 short, with cisregulatoryelements in the 3′ UTR region that are essential for shaping innate immune responses. The derivedamino acidic sequence in silico analysis showed specific binding to human Major Histocompatibility Complex(MHC) Class I and Class II alleles. The role of Ci8 short peptide was investigated in a more evolved immunesystem using human Peripheral Blood Mononuclear Cells (PBMCs) as in vitro model. The biological activities ofthis molecule include the activation of 70 kDa TCR ζ chain Associated Protein kinase (ZAP-70) and T CellReceptor (TCR) Vβ oligo clonal selection on CD4+ T lymphocytes as well as increased proliferation and IFN-γsecretion. Furthermore Ci8 short affects CD4+/CD25high induced regulatory T cells (iTreg) subset selection whichco-expressed the functional markers TGF-β1/Latency Associated Protein (LAP) and CD39/CD73. This paperdescribes a new molecule that modulates important responses of the human adaptive immune system.
|Number of pages||10|
|Publication status||Published - 2018|
All Science Journal Classification (ASJC) codes
- Immunology and Allergy
Vizzini, A., Colombo, P., Schildberg, F. A., Longo, A., Vlah, S., Melis, M. R., Bonura, A., & Gervasi, F. (2018). Ci8 short, a novel LPS-induced peptide from the ascidian Ciona intestinalis,modulates responses of the human immune system. Immunobiology, 223, 210-219.